A 500 MHz High-Sensitivity NMR Spectrometer

描述(由申请人提供)：德克萨斯大学奥斯汀分校申请资金对500兆赫核磁共振波谱仪进行重大升级，以创建一台配备三个射频通道、一个高灵敏度低温探头、z轴脉冲场梯度和重离子去耦合的仪器。这次升级将提供远远超过该大学目前可用的核磁共振能力，目前我们最灵敏的仪器是500 MHz常规探针仪。建议中的仪器将加强用户组的研究计划，这些用户组的共同特征是：1)需要大学现有的核磁共振仪器；2)旨在了解生物化学和生物物理学基本问题的研究；3)培养研究生、本科生和博士后研究员的强大传统；4)美国国立卫生研究院和/或国家科学基金会支持的具有重要核磁共振成分的研究计划。该仪器将促进以下领域的研究：1.RNA和蛋白质结构(Hoffman)2.酶抑制剂设计；代谢途径工程(Liu)3.利用核磁共振分析代谢途径(应用)4.天然产物的合成；生物活性靶标的合成(Martin)5.纳米结构的设计(Krische)6.新型分子和配体的设计(Iverson)7.代谢图谱和细胞表型的表征(Marcotte)拟议的文书得到了大学行政部门的大力支持，将提供6.5万美元的配套资金。此外，该大学还为放置仪器提供实验室空间，并为支持人员提供工资和福利。

DESCRIPTION (provided by applicant): The university of Texas at Austin requests funds for a major upgrade of a 500 MHz NMR spectrometer, to create an instrument equipped with three radiofrequency channels, a high-sensitivity cryoprobe, z-axis pulsed field gradient, and deuterium decoupling. This upgrade will provide NMR capability that is significantly beyond what is currently available at the university, where our most sensitive instrument is currently a 500 MHz conventional-probe instrument. The proposed instrument will enhance the research programs of user groups whose common features are: 1) A need for NMR instrumentation beyond what is currently available at the university; 2) Research directed toward understanding fundamental problems in biochemistry and biophysics; 3) Strong traditions of training graduate and undergraduate students and post-doctoral fellows; 4) NIH- and/or NSFsupported research programs with a significant NMR component. Research in the following areas will be enhanced by the instrument:1. RNA and protein structure (Hoffman)2. Enzyme inhibitor design; engineering of metabolic pathways (Liu)3. Analysis of metabolic pathways using NMR (Appling)4. Synthesis of natural products; synthesis of bioactive targets (Martin)5. Design of nanostructures (Krische)6. Design of novel molecules and ligands (Iverson)7. Metabolic profiling and the characterization of cellular phenotypes (Marcotte). The proposed instrument has the strong endorsement of the university administration, which will provide $65,000 in matching funds. In addition, the university provides laboratory space for housing the instrument, as well as salary and fringe benefits for support personnel.