Pathways to synthetic bacteriophages for phage therapy: Do tRNAs control host range and virulence?

在这个项目中，噬菌体和它们的细菌宿主之间的相互作用将从它们的tRNA含量方面进行研究。本项目的主要重点将是trna在噬菌体中的来源和作用，在宿主范围和毒力方面。将检测宿主和细菌基因组中的tRNA含量，以评估噬菌体与其细菌宿主之间是否存在共同进化的证据。这将与噬菌体trna和细菌密码子的研究相补充，以揭示它们是否编码相同或不同的trna，并检查所讨论的trna的身份。该项目旨在利用克雷伯氏菌、比尔博噬菌体和Aura噬菌体创建一个模型系统。一旦从环境中分离出来，克雷伯菌菌株将感染比尔博和光环噬菌体。然后选择只对两种噬菌体中的一种敏感的菌株。噬菌体trna将被依次敲除，它们的毒力和宿主范围将与它们的“野生型”对应物进行比较。

In this project, the interaction between bacteriophage and their bacterial hosts will be examined in terms of their tRNA content. The main focus of this project will be the provenance and role of tRNAs in phage in terms of host range and virulence. The tRNA content in host and bacterial genomes will be examined to evaluate whether there is evidence for co-evolution between bacteriophage and their bacterial hosts.This will be complemented with a study on bacteriophage tRNAs and bacterial codons to unveil whether they code for the same or different tRNAs, examining the identities of the tRNAs in question.This project will aim to create a model system using Klebsiella sp. and Bilbo and Aura phage. Once isolated from the environment, Klebsiella strains will be infected with Bilbo and Aura phage. The strains susceptible to only one of the two phage will then be selected. Phage tRNAs will be sequentially knocked out, and their virulence and host range will be compared against their "wild type" counterparts.