Biochemical and biophysical mechanisms of macrophage migration and nuclear deformation.

巨噬细胞，特别是组织驻留巨噬细胞（TRM），是至关重要的防御病原体入侵和组织修复通过消除细胞碎片在稳态。尽管迄今为止的许多研究已经阐明了TRM的起源和功能，但对巨噬细胞侵入组织的生化和生物物理机制和动力学，特别是核机制的作用知之甚少。为了解决文献中的这一空白，我们将利用浆细胞迁移模型开发果蝇胚胎，同时建立遗传工具来解开迁移过程中的核力学。使用在体内和体外的方法，数据将收集使用共聚焦和双光子显微镜，我们打算分析核变形及其在有限的迁移的作用。这些见解将揭示脊椎动物巨噬细胞迁移，这将为治疗策略的进步提供信息，包括伤口愈合，癌症，再生和对感染的免疫反应。

Macrophages, specifically tissue resident macrophages (TRMs), are vital in defence against pathogen invasion and tissue repair by eliminating cell debris in homeostasis. Although much of research to date has shed light on the origins and function of TRMs, very little known about the biochemical and biophysical mechanisms and kinetics of macrophage invasion of tissues, especially the role of nuclear mechanisms. To address this gap in the literature, we will utilise the plasmatocyte migration model in developing Drosophila embryos alongside established genetic tools to unravel nuclear mechanics during migration. Using both in vivo and ex vivo methods, data will be collected using confocal and two-photon microscopy where we intend to analyse nuclear deformation and its role in confined migration. These insights will shed light on vertebrate macrophage migration which will inform advancements in treatment strategies including wound healing, cancer, regeneration, and immune response to infections.