Use of zebrafish to study the pathogenesis and evolution of Shigella species

志贺氏菌是一种胞内病原体，也是全球范围内痢疾的主要原因。目前已知四个种：福氏志贺氏菌、宋内志贺氏菌、志贺氏菌痢疾杆菌和志贺氏菌。尽管志贺氏菌的全球系统地理和进化史开始显现，但其潜在的决定因素却知之甚少。斑马鱼是研究体内宿主-病原体相互作用的一个非常稳定的模型系统。莫斯托维实验室最近表明，斑马鱼对模拟人类志贺氏菌感染很有价值。本项目将利用志贺氏菌-斑马鱼感染来比较研究4个志贺氏菌种的致病机理，并研究霍尔特实验室最近的基因组学研究表明的进化现象。

Shigella is an intracellular pathogen and dominant cause of dysentery globally. Four speciesare currently recognized: Shigella flexneri, Shigella sonnei, Shigella dysenteriae and Shigellaboydii. Although the global phylogeography and evolutionary history of Shigella species isstarting to emerge, the underlying determinants are poorly understood. Zebrafish are a wellestablishedmodel system to study host-pathogen interactions in vivo. The Mostowy lab hasrecently shown zebrafish are valuable to model Shigella infection in humans. This project willuse Shigella-zebrafish infection to comparatively investigate the pathogenesis of 4 Shigellaspecies, and study evolutionary phenomena indicated by recent genomics studies from theHolt lab.