Temperature regulation of crop - rhizosphere ecology

“作物通过释放根系分泌物来控制根际相互作用和群落结构。虽然根系分泌物可以吸引有益微生物，但它们也被病原体和植物寄生线虫（ppn）利用。我们的数据表明，温度改变了番茄根系分泌物的组成，改变了与微生物和ppn的相互作用。我们已经开发了转录组和小RNA数据集，用于发育匹配的番茄cv。摇钱树幼苗生长在18、23和28摄氏度，揭示了相当多的基因家族，以及小和长非编码（nc） rna的调控。同样，根分泌物代谢组揭示了温度对化学成分的影响。这代表了一个有价值的实验范例，可用于研究温度对植物生物学和根际相互作用的影响，从基因到生态系统。我们的中心假设是温度调节基因和ncrna将调节根分泌物组成，影响与作物健康和产量相关的根际相互作用。了解单个ncrna在调节这些相互作用中的作用，将有助于开发改良的、适应气候变化的作物，以及了解气候变化背景下的根际生态。该项目将涉及开发表达RNAi / CRISPR-Cas9构建体的转基因番茄植株，以研究基因和ncRNA功能的各个方面。通过LC-Qtof-MS / GC-MS，个体温度调节基因和ncRNAs将与根渗出物化学相关，并通过分子和行为分析与根际相互作用和生态学相关。该项目为成功的候选人提供了一系列令人兴奋的世界级培训机会，涵盖了真正的多学科重点，从计算生物学和生物信息学到基因操作；从根分泌物代谢组学到行为分析和种群结构。该项目还包括一系列国际培训机会。”

"Crop plants manipulate rhizosphere interactions and community structure through the release of root exudates. Whilst root exudates can attract beneficial microbes, they are also exploited by pathogens and plant parasitic nematodes (PPNs). Our data indicate that temperature modifies tomato root exudate composition, altering interactions with microbes and PPNs. We have developed transcriptomic and small RNA datasets for developmentally matched tomato cv. Moneymaker seedlings grown at 18, 23 and 28 degrees C, revealing considerable regulation of diverse gene families, alongside small and long non-coding (nc)RNAs. Likewise, root exudate metabolomes reveal considerable variation in chemical composition as a factor of temperature. This represents a valuable experimental paradigm, which can be used to study the impact of temperature on plant biology and rhizoshpere interactions, from genes to ecosystems. Our central hypothesis is that temperature-modulated genes and ncRNAs will regulate root exudate composition, influencing rhizosphere interactions relevant to crop health and yield. Understanding the role of individual ncRNAs in regulating these interactions will underpin efforts to develop improved, climate-resilient crops, and to understand rhizosphere ecology in the context of a changing climate. This project will involve the development of transgenic tomato plants expressing RNAi / CRISPR-Cas9 constructs to study aspects of gene and ncRNA function. Individual temperature-regulated genes and ncRNAs will be related to root exudate chemistry using LC-Qtof-MS / GC-MS, and rhizosphere interactions and ecology, through molecular and behavioural assays. This project affords the successful candidate a range of exciting, world-class training opportunities, spanning a genuinely multidisciplinary focus, from computational biology and bioinformatics to gene manipulation; from root exudate metabolomics to behavioural assays and population structure. This project also includes a range of international training opportunities."