CORE--TISSUE CULTURE/HYBRIDOMA

位于解剖学/细胞生物学系的组织培养/杂交/分子模块将提供组织和器官培养的集中设施以及经验丰富的研究助理。研究助理将帮助那些在组织培养方面缺乏专业知识的研究人员开发与其研究相关的实验方法。此外，她将协助研究人员生产单克隆抗体，并进行常规分子技术。需要使用模块的项目将提交给模块联合主任（Kurpakus-Whater博士）进行初步咨询。一旦获得批准，项目将由提出工作的PI或其实验室人员在模块研究助理的直接监督和帮助下进行。新的专用空间和设备 大大扩展和增强了该模块的功能。缺乏分子生物学、组织/器官培养或单克隆抗体生产所需技术经验的研究者将严重依赖研究助理的设备和可用性，为其项目提供技术咨询。了解所涉技术方面的调查人员将利用该设施加强他们自己的研究，并开始试验性研究项目。因此，这个中央核心设施将继续提供一个合理的，具有成本效益的机制，以促进和加强众多NEI资助的研究项目。

The Tissue Culture/Hybridoma/Molecular Module, located in the Department of Anatomy/Cell Biology will provide a centralized facility for tissue and organ culture and an experienced research assistant. The research assistant will be available to help investigators who have little expertise in tissue culture to develop experimental methods relevant to their studies. In addition, she will assist investigators with the production of mAbs and in carrying out routine molecular techniques. Projects proposed requiring Module use will be presented to the Module co-director (Dr. Kurpakus-Wheater) for initial consultation. Once approved, the projects will be undertaken by either the PI proposing the work or personnel from his/her laboratory under direct supervision and with the help of the Module research assistant. The newly dedicated space and equipment for molecular studies has greatly expanded and enhanced the functions of this module. Those investigators who lack experience in the technology required for molecular biology, tissue/organ culture, or mAb production will rely heavily on the equipment and availability of the research assistant for technical advice to their projects. Those investigators knowledgeable in the technical aspects involved will use the facility to enhance their own studies and to begin pilot research projects. This centralized Core facility will thus continue to provide a reasonable, cost efficient mechanism to facilitate and enhance numerous NEI funded research projects.