Plasma Markers of Dietary Intervention to Delay Cogniti

我们建议进行一项饮食干预的试点研究，以延缓老年妇女的认知能力下降。由于生物标志物可能比饮食问卷更能反映组织的营养物质生物可利用性，因此我们建议前瞻性地检查各种抗氧化剂的血浆标志物（α-胡萝卜素、β-胡萝卜素、叶黄素/玉米黄质、番茄红素、β-隐黄质、α-生育酚和γ-生育酚）和同型半胱氨酸、叶酸和维生素B6和B12以及它们与护士健康研究（NHS）的老年参与者中认知能力下降的关联。NHS始于1976年，覆盖了121，700名30至55岁的女性; 1989-1990年， 采集了32，000份血液样本。从1995年到2001年，大约有19，000多名70岁或以上的无中风女性完成了基线电话认知访谈，包括测量一般认知能力、情景记忆、类别流畅性和注意力的各种测试。我们已经进行了第二波认知测试，第三次评估正在进行中，从而使我们能够前瞻性地测量认知功能相对于基线的变化。几条证据表明，所提出的标志物的单一血浆测量值很好地代表了长期状态，并且越来越多的证据表明， 早年的接触对于老年人的神经保护可能特别重要。我们将利用来自NHS的乳腺癌和结肠癌既往巢式病例对照的对照， 结肠息肉、透镜混浊和心脏病，其血浆抗氧化剂、同型半胱氨酸和B族维生素以及认知功能的测量已经完成（同型半胱氨酸/B族维生素n=567，血浆抗氧化剂n= 724）。我们将能够检查基线性能和纵向变化的认知功能，在每一个测试随着时间的推移与不同的血浆水平组，使用广义估计方程的重复测量与随机效应。首席研究员是一个新的，年轻的研究人员，和拟议的补助金将是重要的，以促进她的职业生涯在老龄化研究。重要的是，拟议拨款的结果将为未来的大规模综合研究奠定基础，以检查饮食干预延迟认知功能。

We propose to conduct a pilot study of dietary interventions to delay cognitive decline in older women. Since biomarkers may better reflect nutrients bioavailable to tissues than dietary questionnaires, we propose to prospectively examine plasma markers of a wide array of antioxidants (alpha-carotene, beta-carotene, lutein/zeaxanthin, lycopene, beta-cryptoxanthin, alpha-tocopherol and gamma-tocopherol) and homocysteine, folate and vitamins B6 and B12 and their association with cognitive decline among older participants of the Nurses Health Study (NHS). The NHS began in 1976 among 121,700 women aged 30 to 55; in 1989-1990, over 32,000 blood samples were collected. From 1995-2001, approximately 19,000+ women free of stroke and aged 70 or above completed a baseline telephone cognitive interview consisting of a variety of tests measuring general cognitive ability, episodic memory, category fluency and attention. A second wave of our cognitive tests has been administered and the third assessments are ongoing, thus allowing us to prospectively measure change in cognitive function from baseline. Several lines of evidence establish that a single plasma measure of the proposed markers well-represent long-term status and accumulating evidence indicates that earlier life exposures may be particularly important for neuroprotection in older ages. We will utilize controls from previous nested case-controls from the NHS of breast and colon cancer, colon polyps, lens opacities and heart disease for whom measurements of plasma antioxidants, homocysteine and B vitamins and cognitive function have already been completed (n=567 for homocysteine/B vitamins and 724 for plasma antioxidants). We will be able to examine both the baseline performance and longitudinal change in cognitive function on each test over time in relation to various plasma level groups, using generalized estimating equations of repeated measures with random effects.The Principal Investigator is a new, young researcher, and the proposed grant will be important in facilitating her career in aging research. Importantly, results from the proposed grant will lay the groundwork for a future, large-scale comprehensive study to examine dietary interventions to delay cognitive function.