Optimize tools to study gene functions in pest invertebrates with a focus on aphids and whiteflies

该项目是Saskia Hogenhout（英国诺威奇John Innes中心）、Grant Hughes（英国利物浦医学院）和Marcus Guest（英国Jealotts Hill先正达公司）实验室的三方合作，旨在开发蚜虫Myzus persicae和烟草粉虱Bemisia tabaci基因敲除技术。获得这些技术不仅对于获得关于这些昆虫如何与植物建立密切相互作用以及它们如何有效传播病毒的基本知识很重要，而且对于确定农用化学品的新靶点也很重要，以控制对蜜蜂等有益无脊椎动物无害的半足类害虫。该项目有以下三个目的：(1)挖掘和注释桃蚜和烟粉虱基因组中特定通路的基因；(2)筛选适合桃蚜和烟粉虱种系的配体；(3)产生桃蚜和烟粉虱基因敲除

This project is a three-way collaboration of the laboratories of Saskia Hogenhout (John Innes Centre, Norwich, UK), Grant Hughes (Liverpool School of Medicine (LSTMed), UK) and Marcus Guest (Syngenta, Jealotts Hill, UK) to develop gene knock-out technologies for the aphid Myzus persicae and the tobacco whitefly Bemisia tabaci. Access to these technologies will be important not only for generating fundamental knowledge on how these insects establish intimate interactions with plants and how they efficiently transmit viruses, but also for identifying new target sites for agrochemicals to control hemipteran pests that are not damaging for beneficial invertebrates, such as bees.The project has the following three aims: (1) Mine and annotate genes of specific pathways in the M. persicae and B. tabaci genomes; (2) Identify a suitable ligand to target the M. persicae and B. tabaci germlines; (3) Generate gene knock outs in M. persicae and B. tabaci