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Imaging Transcriptionally Active Genes by Electron Micro

Imaging Transcriptionally Active Genes by Electron Micro
通过电子显微镜对转录活性基因进行成像
批准号：
6837257
负责人：
Richard D Leapman
金额：
$0.0万
依托单位：
OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/6837257
关键词：
bioimaging /biomedical imaging charge coupled device camera electron microscopy fluorescent dye /probe genetic transcription image processing immunofluorescence technique nucleic acid structure phosphorus tissue /cell culture

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中文摘要
翻译
能量过滤成像被用于绘制DNA在细胞核特定区域的组织，通过对培养细胞中的磷分布进行成像，这些细胞被荧光标记的抗体标记，以揭示转录活性基因的位置。使用配备柱后成像滤光片和1024x1024像素冷却慢扫描CCD相机的FEI CM120电子显微镜对塑料嵌入切片进行分析。元素图是通过仔细减去边缘前的背景强度，从磷L23核心边缘以下和上方记录的图像中计算出来的。目前正在研究高压冷冻技术，以确定是否可以从细胞核内的DNA纤维中获得改进的超微结构，从而更高精度地定位转录位点。
英文摘要
Energy-filtered imaging is being used to map the organization of DNA in specific regions of nuclei by imaging the phosphorus distributions in cultured cells that are labeled with fluorescently tagged antibodies to reveal the location of transcriptionally active genes. Plastic embedded sections are analyzed with an FEI CM120 electron microscope equipped with a post-column imaging filter and a 1024x1024 pixel cooled slow-scan CCD camera. Elemental maps are computed from images recorded below and above the phosphorus L23 core edge by careful subtraction of the pre-edge background intensity. High-pressure freezing techniques are being investigated to determine whether improved ultrastructure can be obtained from DNA fibers within the nucleus in order to localize the transcription sites with higher precision.
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SUBUNIT ORGANIZATION AND STRUCTURE OF LON PROTEASE
EXPERIMENTS WITH A HIGH RESOLUTION FIELD EMISSION STEM
High Pressure Freezing Of Cultured Neurons
Mass Mapping of Macromolecular Assemblies
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