Development of a Cytomegalovirus Vaccine

超出所提供的空间。 人类巨细胞病毒（HCMV），一个成员的疱疹病毒家族，是导致出生缺陷的主要病毒原因，并导致显着的发病率和死亡率在免疫抑制的个人。非常需要预防巨细胞病毒（CMV）相关疾病的疫苗，其中在达到生育年龄之前对妇女进行免疫接种是至关重要的。我们研究的主要目的是利用小鼠模型来开发一种疫苗，该疫苗将提供杀菌免疫力，并完全保护免受急性和潜伏CMV感染。该策略是使用DNA疫苗接种与灭活病毒免疫相结合的技术，以确定哪种鼠巨细胞病毒（MCMV）基因产物和疫苗给药途径的组合将产生最有力的和保护性的免疫应答，以对抗随后的胃肠外或粘膜病毒攻击。我们已经完成了一些试点项目，并在实现该项目目标方面取得了重大进展。我们最重要的成就是，我们已经开发出一种疫苗方法，完全保护BALB/c小鼠免受随后的强毒病毒全身感染。在这项资助申请中，我们建议实现以下三个目标：1。确定双重免疫对粘膜攻击的保护效力; 2.确定DNA免疫途径是否影响保护; 3.优化疫苗接种方案。这项研究的长期目标是阐明成功接种疫苗的基本原则，并将这些信息应用于人类疫苗的开发。性能现场=

EXCEED THE SPACE PROVIDED. Human cytomegalovirus (HCMV), a member of the herepesvirus family, is the leading viral cause of birth defects and causes significant morbidity and mortality in immunosuppressed individuals. There is a great need for vaccines that will prevent cytomegalovirus (CMV) associated disease, with the immunization of women before reaching childbearing age being of paramount importance. The major objective of our research is to utilize the murine model to develop a vaccine that will provide sterilizing immuniity and protect fully against both the acute and latent CMV infection. The strategy is to use the technique of DNA vaccination coupled with immunization with inactivated virus to determine which combination of murine cytomegalovirus (MCMV) gene products and route of administration of vaccine will generate the most vigorous and protective immune response against a subsequent parenteral or mucosal viral challenge. We have already completed a number of pilot projects and have made significant progress towards achieving the goals of this project. Our most important accomplishment is that we have developed a vaccine approach that completely protects BALB/c mice from subsequent systemic infection with virulent virus. In this grant application, we propose to accomplish the following 3 aims: 1. Determine the protective efficacy of the dual immunization against mucosal challenge; 2. Determine whether the route of DNA immunization affects protection; and 3. Optimize the vaccination protocol. The long range goals of this research are to elucidate the underlying principles of successful vaccination and apply this information towards the development of a human vaccine. PERFORMANCE SITE ========================================Section End===========================================