Extended DNA amplification with thermostable chaperons

描述（由申请人提供）：该项目的目标是开发一种通用的方法来提高用于DNA和RNA扩增、测序、限制性内切分析和连接的酶的稳定性和耐久性。有待改进的酶包括DNA聚合酶、拓扑异构酶、逆转录酶和连接酶。如果这一目标能够实现，可能会对依赖DNA测序、扩增和RT-PCR过程的临床、法医和基础科学领域产生广泛影响。为了做到这一点，由来自超嗜热细菌的伴侣介导的蛋白质的外部稳定和重折叠将被适应纳入与DNA修饰酶的反应混合物中。

DESCRIPTION (provided by applicant): The goal of this project is to develop a general method of improving the stability and durability of enzymes used in DNA and RNA amplification, sequencing, restriction analysis and ligation. The enzymes to be improved include DNA polymerases, topoisomerases, reverse transcriptases and ligases. In the event this goal can be achieved, broad impacts may result in the areas of clinical, forensic and basic science that rely on DNA sequencing, amplification and RT-PCR processes. To do this, extrinsic stabilization and refolding of proteins mediated by chaperones from hyperthermophiles will be adapted for incorporation into reaction mixtures with DNA modifying enzymes.