Engineering T cells with enhanced ligand discriminatory powers

表面受体的区分能力量化了其区分低亲和力和高亲和力配体的能力。T细胞受体已经进化出增强的区分自身和外来抗原配体的能力，但其机制尚不清楚。在这里，我将使用合成生物学方法来理解这些机制，通过（1）工程化靶向不同T细胞表面蛋白的双特异性试剂以识别调节天然TCR的识别能力的那些蛋白，以及通过（2）工程化合成增强识别受体（EDRs）。该项目解决了“合成生物学”的BBSRC响应模式优先级，其科学范围包括工程细胞与新的生物部件和系统。

The discriminatory power of a surface receptor quantifies its ability to distinguish between lower-affinity and higher-affinity ligands. The T cell receptor has evolved enhanced discriminatory powers to distinguish between self and foreign antigen ligands but the mechanisms are unclear. Here, I will use a synthetic biology approach to understand these mechanisms by (1) engineering bispecific reagents that target different T cell surface proteins to identify those that regulate the discrimination power of native TCRs and by (2) engineering synthetic enhanced discriminatory receptors (EDRs).This project addresses the BBSRC Responsive Mode Priority of "Synthetic Biology", whose scientific scopeincludes engineering cells with novel biological parts and systems.