Cell culture animal models for SARS-CoV

描述（由申请人提供）：2003年春天，东南亚开始爆发不明病因，并迅速蔓延到其他几个国家。这种疾病被称为严重急性呼吸窘迫综合征或SARS，是由世卫组织和地方当局协调的大规模公共卫生努力确定和控制的。这种疾病的病原体被确定为一种新的冠状病毒株，并命名为SARS-CoV。为了获得关于病毒复制、发病机理和诱导的免疫应答的额外知识，需要动物和细胞培养模型。本申请中描述的实验旨在建立1）使用原代呼吸道上皮细胞的SARS-CoV感染的体外组织培养模型，2）使用野生型以及免疫缺陷小鼠品系的SARS-CoV感染的小鼠模型和3）未知功能的SARS-CoV ORF的结构/功能分析系统。成功完成本申请中概述的目标将建立研究SARS-CoV细胞生物学和发病机制的系统。

DESCRIPTION (provided by applicant): In the spring of 2003, an outbreak of unknown etiology began in South East Asia and quickly spread to several other countries. The disease, termed severe acute respiratory distress syndrome or SARS, was identified and contained by a massive public health effort orchestrated by the WHO and local authorities. The etiological agent of this disease was identified as a new strain of coronavirus, and given the name SARS-CoV. In order to gain additional knowledge as to the replication, pathogenesis and immune responses induced by the virus, animal and cell culture models are needed. The experiments described in this application are aimed at establishing 1) in vitro tissue culture models for SARS-CoV infection using primary respiratory epithelial cells 2) a mouse model for SARS-CoV infection using wild type as well as immunodeficient mouse strains and 3) structure/function analysis systems for SARS-CoV ORFs of unknown function. Successful completion of the aims outlined in this application will establish systems for investigating the cell biology and pathogenesis of SARS-CoV.