Chemistry Core

核心A -“化学核心”。Chemistry Core“支持生产特殊样品， 是本项目各试验项目所需要的。有三个组成部分： （一）.根据需要进行蛋白质和突变体的过表达和纯化。这项工作将在 AECOM为纽约财团开发的蛋白质生产设施 结构基因组学项目，由NIGMS资助。 (2)含有位点特异性掺入的稳定同位素（13 C、15 N、2 H）的蛋白质样品的合成。这 将在洛克菲勒大学进行，由汤姆·缪尔指导。他的实验室一直是 蛋白质半合成技术，表达蛋白质连接，它允许任何靶蛋白质， 在体外由两个或更多个片段组装，其可以是重组或合成来源的。 (3)根据需要合成掺入稳定同位素（13 C，15 N，2 H）位点的小分子 具体来说小分子合成将在碳水化合物化学，天然产物进行 新西兰下赫特工业研究有限公司加工组。Richard Furneanx博士， 碳水化合物化学小组将监督这项工作。

Core A - 'The Chemistry Core'. The Chemistry Core' supports the production of specialized samples that are needed by the various experimental Projects of the Program Project. There are three components to this: (1). The overexpression and purification of proteins and mutants as required. This work will be carried out at the protein production facility at the AECOM that has been developed for the New York Consortium Structural Genomics Project, funded by NIGMS. (2) The synthesis of protein samples containing site-specific incorporated stable isotopes (13C, 15N, 2H). This will be carried out at Rockefeller University under the direction of Tom Muir. His lab has been a pioneer of the protein semisynthesis technique, Expressed Protein Ligation, which allows any target protein to be assembled in vitro from two or more segments, which can be recombinant or synthetic in origin. (3) The synthesis of small molecules as required that incorporate stable isotopes (13C, 15N, 2H) site specifically. Small molecule synthesis will be performed at Carbohydrate Chemistry, Natural Products Processing Group, Industrial Research Limited, Lower Hutt, New Zealand. Dr. Richard Furneanx, director of the Carbohydrate Chemistry Group, will oversee this effort.