Core--Microscopy

核心将提供电子和光学显微镜成像，这对实现各个项目的目标至关重要。细胞内定位的蛋白质，核质运输的转录因子，和肌球蛋白丝的成像是关键，以测试本计划的假设，并将需要广泛使用共聚焦显微镜和电子显微镜。核心将为个别研究人员提供培训，调整和校准这些仪器，协助准备标本，并监督实验和维护的时间安排。核心包括一个由当地显微镜专家组成的小组，他们将进行培训并实施最新的升级和创新。核心员工将确保该计划 有效和高效地利用这些资源。显微镜的共同核心带来了其他好处，包括共享协议和试剂，交易正在进行的实验信息，以及培养从事不同项目的个人之间的同志情谊，因为他们使用共同的设施和方法来研究相关问题。

The Core will provide electron and light microscopy imaging that is essential to accomplishing the goals of the individual projects. The intraceltular localization of proteins, nucleo-cytoplasmic transport of transcription factors, and imaging of myosin filaments are key to testing the hypotheses of this Program, and will require extensive use of confocal microscopy and electron microscopy. The core will provide training for individual investigators, align and calibrate these instruments, assist with preparation of specimens, and oversee scheduling of experiments and maintenance. The core includes a panel of local microscopy experts, who will conduct training and implement the latest upgrades and innovations. The Core staff wilt ensure that the Program gets effective and efficient use of these resources. A common core for microscopy brings with it other benefits in terms of sharing of protocols and reagents, trading of information on experiments in progress and nurturing a sense of camaraderie between individuals working on different projects, as they use common facilities and methodology to study related issues.