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CORE--MICROSCOPY AND IMMUNOHISTOCHEMISTRY

CORE--MICROSCOPY AND IMMUNOHISTOCHEMISTRY
核心——显微镜和免疫组织化学
批准号:
7074742
负责人:
JAMES RICHARDSON
金额:
$37.42万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至

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中文摘要
翻译
我 描述(由申请人提供): 运动核心设施将提供两种不同的方法,自愿轮运行, 运动神经的低频紧张性起搏,以增强肌肉的收缩活动, 完整的老鼠这两个过程都与内源性基因的改变有关, 表达与纤维类型转化一致。这些程序以前是 在野生型和转基因动物中开发、测试并成功使用, 在德州大学西南分校的巴塞尔-杜比博士的指导下此外,我们亦会研究 分离的骨骼肌功能,以研究等长和等张收缩功能, 小鼠骨骼肌
英文摘要
I DESCRIPTION (provided by applicant): The Exercise Core Facility will provide two different approaches, voluntary wheel running and low-frequency tonic pacing of the motor nerve, to augment contractile activity in muscles of intact mice. Both of these procedures are associated with changes in endogenous gene expression consistent with fiber type transformation. These procedures have previously been developed, tested and successfully used in wild type and transgenic animals under the supervision of Dr. Bassel-Duby at UT Southwestern. In addition, we will perform studies on isolated skeletal muscle function to study the isometric and isotonic contractile function of mouse skeletal muscle.
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