High Trapping Efficiency Ion Trap Mass Spectrometer

描述（由申请人提供）：本提案的目的是证明高捕集效率离子阱的可行性。该装置可用于离子阱质谱仪，包括离子阱质谱仪（ITMS）和混合四极离子阱飞行时间质谱仪（QitTOF）。提出的离子阱将使用一个特殊构造的分裂环连接到一对匹配的射频电压源，以提供一个四极场，可以在2D（线性阱）和3D（保罗阱）模式之间进行电子切换。使用线性阱模式捕获（注入）离子，预计捕获效率比3D离子阱提高10到20倍。在离子被捕获并在陷阱内冷却后，射频电子设备可以切换回传统的3D离子陷阱模式来扫描和喷射离子。因此，捕获效率的提高将导致灵敏度的显著提高。这种离子阱的3D扫描和弹出模式将提供一个紧密的输出离子束，为附加组件（如TOF MS或外部破碎设备）提供一个简单的接口。这种配置可以应用于当前和未来使用离子阱的分析仪器，以及其他离子阱应用，使人们能够充分利用2D线性阱和3D保罗阱的优点。

DESCRIPTION (provided by applicant): The objective of this proposal is to demonstrate the feasibility of a high trapping efficiency ion trap. This device can be used on ion trap based mass spectrometers including ion trap mass spectrometers (ITMS) and hybrid quadrupole ion trap time-of-flight mass spectrometers (QitTOF). The proposed ion trap will use a specially constructed split ring connected to a pair of matched RF voltage sources, to provide a quadrupole field that can be switched between 2D (linear trap) and 3D (Paul trap) modes electronically. Using the linear trap mode for trapping (injecting) ions, a projected 10 to 20 fold improvement in trapping efficiency can be achieved versus a 3D ion trap. After the ions are trapped and cooled inside the trap, the RF electronics can be switched back to the conventional 3D ion trap mode to scan and eject the ions. The trapping efficiency improvement would thus result in a significant improvement in sensitivity. The 3D scan and eject mode of this ion trap would provide a tight output ion beam, making for a simple interface to additional components such as a TOF MS or external fragmentation device. This configuration could be applied to current and future analytical instruments that use an ion trap, as well as to other ion trap applications, allowing one to take advantage of the best of both the 2D linear trap and the 3D Paul trap. Development of the proposed device could improve the performance of several analytical tools for proteomics research. For low abundant protein samples, it could provide much better sensitivity, with lower detection limits. The impact of ion trap technology development has been seen in many areas of life science research, and this proposed work should help continue this ion trap evolution. This work will not only benefit ion trap related mass spectrometer development, but will also expand our knowledge base of ion traps in general.