Neonatal Alcohol Exposure and 1H-NMR Imaging in Rats

描述(申请人提供)：目前的建议的目的是用高分辨率磁角自旋质子磁共振波谱(HR-MAS 1H-核磁共振)检测体外脑神经化学，以及使用磁共振成像(MRI)在体内检测新生大鼠酒精暴露后的脑结构。这一时期对应于人脑发育的第三个三个月，被称为“脑生长突发期”。在此期间接触酒精对大脑和行为都有广泛的影响。有人建议，上述成像技术将提供一个独特的机会，以检查酒精暴露后大脑发育的结构和神经化学方面。受试者将在新生儿期接触酒精，然后在不同年龄(PN16、PN28或PN63)从不同区域采集组织样本，使用HR MAS 1H-MRS进行神经化学分析。其他受试者将在PN28使用磁共振成像进行活体成像。使用这些技术，人们能够评估在发育过程中新生儿酒精暴露后可能改变的大脑局部代谢物的变化。人们认为，这项工作最终将有助于临床医生诊断和治疗胎儿酒精影响。

DESCRIPTION (provided by applicant): The purpose of the current proposal is to examine brain neurochemistry in vitro with high-resolution magicangle-spin proton magnetic resonance spectroscopy (HR-MAS 1H-NMR) as well as brain structure using magnetic resonance imaging (MRI) in vivo after neonatal alcohol exposure in the rat. This period corresponds to the third trimester of human brain growth and is known as the "brain growth spurt". Exposure to alcohol during this period has widespread effects on both the brain and behavior. It is proposed that the above mentioned imaging techniques will provide a unique opportunity to examine structural and neurochemical aspects of brain development following alcohol exposure. Subjects will be exposed to alcohol during the neonatal period, then at various ages (PN16, PN28, or PN63) tissue samples will be taken from various regions for analyses of neurochemistry using HR MAS 1H-MRS. Other subjects will be imaged in vivo using MRI at PN28. Using these techniques, one is able to assess changes in regional brain metabolites that may be altered following neonatal alcohol exposure during development. It is thought that ultimately this work will aid clinicians in diagnoses and treatment of Fetal Alcohol Effects.