CORE--MOLECULAR VIROLOGY

核心D描述(由申请人提供)：分子病毒学核心是一个必要的 DUMC CFAR的组成部分，作为临床和基础研究之间的资源纽带。这一核心由BSL-3病毒学设施、DNA序列分析设施和分子构建设施组成，以满足参与艾滋病毒和相关机会性感染基础和临床转化研究的杜克大学研究人员日益增长的需求。分子病毒学核心的总体目标是：1.促进分子病毒学分析、DNA测序和病毒学分析在CFAR实验室通过共享资源以具有成本效益的方式实施和验证。2.为人类特定基因在HIV-1或机会性感染的复制和发病机制中的作用的RNA干扰分析提供最先进的支持。3.促进学术界和工业界的跨学科合作，以进一步改进核心提供的技术，并提供可促进合作的坚实资源。4.向CFAR科学家提供现有和新开发的病毒学方法方面的教育和培训机会。为了实现这些目标，分子病毒学核心致力于A.)通过多个QA/QC程序和B。)建立基于CFAR调查人员当前和未来科学需求的病毒学资源。

CORE D DESCRIPTION (provided by applicant): The Molecular Virology Core is an essential component of the DUMC CFAR as a resource link between clinical and basic research. This core is comprised of a BSL-3 Virology Facility, a DNA Sequence Analysis Facility and a Molecular Construct Facility to serve the growing needs of Duke investigators involved in basic and clinical translational research on HIV and associated opportunistic Infections. The overall aims of the Molecular Virology Core are: 1. To facilitate the implementation and validation of molecular virology assays, DNA sequencing, and virologic assays in CFAR laboratories through shared resources in a cost efficient manner. 2. To provide state-of-the-art support for RNA interference analysis of the role of specific human genes in the replication and pathogenesis of HIV-1 or opportunistic infections. 3. To stimulate interdisciplinary collaborations within academia and industry to further improve the technology offered by the Core and to provide a solid resource on which collaborations can be fostered. 4. To provide education and training opportunities to CFAR scientists in existing and newly developed virologic methodologies. In order to achieve these aims, the Molecular Virology Core is committed to A.) maintaining a high quality of reagents and data through multiple QA/QC procedures and B.) establishing a virologic resource that is based on current and future scientific needs of CFAR investigators.