Core--DNA and Peptide Synthesis

寡核苷酸、多肽和蛋白质测序是现代分子医学和生物学的重要工具。获得这些工具对于调查和诊断与转化细胞状态和许多传染病有关的基因改变至关重要。DNA/多肽共享资源为犹他大学癌症中心成员和研究社区的其他人提供可靠、快速和负担得起的合成DNA和RNA(寡核苷酸)、多肽和蛋白质测序。 寡核苷酸用于DNA测序、聚合酶链式反应、定点突变，以及用作识别遗传信息的探针。修饰的寡核苷酸已被用于反义实验和具有治疗可能性的基因沉默实验。合成肽被用作探索蛋白质特定区域的功能或产生免疫试剂的直接手段。蛋白质测序用于确认蛋白质的特性或起始位置，或识别感兴趣蛋白质中的氨基酸序列区域。该设施以快速、高效的方式提供这些服务，将各个实验室的成本和风险降至最低。如果癌症中心成员开发需要它们的试剂或技术，它还提供使用仪器的途径。 最后，该设施为使用各种DNA/多肽技术的癌症中心成员提供信息来源。

Oligonucleotides, peptides and protein sequencing are essential tools of modem molecular medicine and biology. Access to these tools is essential for investigation and diagnosis of genetic alterations linked to transformed cell states and many infectious diseases. The DNA/Peptide Shared Resource provides a dependable, rapid and affordable supply of synthetic DNA and RNA (oligonucleotides), peptides and protein sequencing for cancer center members and others in the research community at the University of Utah. Oligonucleotides are used in DNA sequencing, as polymerase chain reaction primers, for site-directed mutagenesis, and as probes for the identification of genetic information. Modified oligonucleotides have been used for antisense experiments and gene silencing experiments with therapeutic possibilities. Synthetic peptides are used as a direct means of exploring the function of, or to generate ilmnunological reagents against, specific regions of proteins. Protein sequencing is used to confirm the identity or start sites of proteins or to identify regions of amino acid sequence in proteins of interest. This facility provides these services in a fast, efficient manner that minimizes the cost and risk to individual laboratories. It also provides access to instrumentation should cancer center members develop reagents or techniques requiring them. Finally, the facility serves as an information source for cancer center members using a wide variety of DNA/Peptide techniques.