CORE--CELL AND IMAGING

该核心将协调并提供：1.人志愿者PMNs; 2.人内皮细胞系（肺微血管hMVEC）的批次; 3.成像和光学实验设备（FRET、3D重建、多通道成像和细胞术）;和4.有效利用标记的一抗和二抗的易腐库存。目前，核心配备了两个数字去卷积显微镜和贝克曼库尔特流式细胞仪和完整的组织培养能力。这两款示波器都配备了多达4个颜色通道的活细胞或固定细胞3D成像。核心由在原代人类细胞培养和各种成像方法的各个方面都具有丰富经验的人员组成。核心将积极走向未来（2-4年），1。通过将数据与国家数据库（主要是全球环境展望项目）连接起来;在需要之前扩展自己的分析方法库，3.与TCG内的其他核心分析工作（NMR，MS，微阵列）相关联。

This core will coordinate and provide: 1. human volunteer PMNs; 2. batches of human endothelial cell lines (pulmonary microvascular hMVEC); 3. imaging and optical experiment facility (FRET, 3D reconstruction, multichannel imaging and cytometry); and 4. efficient use of a perishable inventory of labeled primary and secondary antibodies. At present the Core is equipped with two digital deconvolving microscopes and Beckman Coulter flow cytometer and full tissue culture capabilities. Both scopes are equipped to perform live or fixed cell 3D imaging in upto 4 color channels. The Core is staffed by personnel who are highly experienced in all aspects of primary human cell culture and various imaging methodologies. The core will actively move into the future (2-4 years), 1. by linking data to national databases (primarily the GEO project) and 2. expanding its own repertoire of analytical methods before the need arises, 3. Linking to other core analytical efforts (NMR, MS, microarrays) within the TCG.