ABI QSTAR XL Q-Q TOF Mass Spectrometer

描述(申请人提供)：多糖可以修饰蛋白质或脂质，具有多种功能，从调节细胞外信号到调节细胞与细胞之间的直接相互作用，或者作为病原体及其毒素的结合部位。为了充分理解多糖的生物学作用，有必要确定其结构。有几种方法可用，每种方法都有其优点和缺点。核磁共振波谱代表了结构分析的金标准，因为它给出了每个单糖的连接和异常构型。然而，很少有足够纯度的材料来进行核磁共振分析。气相色谱-质谱法检测部分甲基化的阿迪醇乙酸酯也有类似的局限性。高pH阴离子交换层析与脉冲安培检测只给出保留时间。质谱学在确定多糖结构方面发挥着越来越重要的作用。它可以应用于多聚糖的混合物。根据多糖的分子量可以简单地推断出多糖的单糖组成。糖苷键的断裂给出了糖链的分支模式，而跨环裂解得到了关于连接位置的信息。在这里，我们申请资助ABI QSTAR XL QqTOF质谱仪和MALDI来源，用于表征多糖和糖肽的结构。该仪器将支持资金雄厚的不同研究人员的研究目标，他们对多糖的生物学意义有着共同的兴趣。所要求的仪器将被安置在加州大学圣迭戈分校的糖类技术核心中。Core成立于10年前，专门研究多糖和糖蛋白的特性，并为圣地亚哥地区和其他地方的糖生物学家提供服务。主任(布拉德利·海斯博士)在使用质谱学方面有丰富的经验。他开发了使用ESI质谱仪确定O-糖基化位点的方法，并实施了使用MALDI-TOF质谱仪分析过甲基化多聚糖混合物的方法。他将负责咨询调查人员，实施新的分析多糖的MS方法，确保仪器正常操作和护理，并根据要求帮助解释数据。Glycotech Core内部有足够的空间和电气服务，可容纳所需的仪器。

DESCRIPTION (provided by applicant): Glycans can decorate either proteins or lipids and serve a variety of functions from modulating extra cellular signals to mediating direct cell-cell interactions or serving as binding sites for pathogenic organisms and their toxins. In order to fully comprehend the biological roles of glycans, it is necessary to determine their structures. Several methodologies are available and each has its strengths and weaknesses. NMR spectroscopy represents the gold standard in structural elucidation since it gives the linkage and anomeric configuration of each monosaccharide. However, rarely is there enough material of sufficient purity for NMR analysis. Gas chromatography with mass spectrometric detection of partially methylated alditol acetates suffers from similar limitations. High pH anion exchange chromatography with pulsed amperometric detection gives only retention times. Mass spectrometry is playing an increasing important role in defining glycan structure. It can be applied to mixtures of glycans. The monosaccharide composition of a glycan can be deduced simply from the molecular weight of the glycan. Fragmentation of glycosidic bonds gives the branching pattern of the glycan and cross-ring cleavages yields information about linkage position. Here we request funding for an ABI QSTAR XL QqTOF mass spectrometer and MALDI source for the structural characterization of glycans and glycopeptides. This instrument will support the research aims of a diverse group of well-funded investigators with a common interest in the biological significance of glycans. The requested instrument will be housed in the UCSD Glycotechnology Core. Established 10 years ago, the Core specializes in the characterization of glycans and glycoproteins and serves glycobiologists both in the San Diego area and elsewhere. The Director (Dr. Bradley Hayes) has considerable experience using mass spectrometry. He has developed methods for determining sites of O-glycosylation using ESI mass spectrometry and has implemented methods for analyzing mixtures of permethylated glycans using MALDI-TOF mass spectrometry. He will be responsible for consulting with investigators, for implement new MS methods for the analysis of glycans, for ensuring the instrument is properly operated and cared for and for helping with the interpretation of data, as requested. Sufficient floor space and electrical service is available within the Glycotechnology Core to accommodate the requested instrument.