LSM 510 Confocal Microscope

描述（由申请人提供）：我们要求Zeiss LSM 510-Meta共聚焦显微镜，用于对各种细胞和组织进行灵敏的荧光成像。该显微镜将为来自约翰霍普金斯大学医学院多个部门的8个主要用户和至少50个次要用户提供服务。用户需要的显微镜，提供最高的灵敏度和空间分辨率，最小的串扰和渗漏，高速度和吞吐量，光栅扫描和波长分析的FRET和FRAP的灵活性，以及强大的软件图像处理和分析。Zeiss 510共聚焦的特点包括：多跟踪技术，用于快速和精确的像素定位; Meta多通道光谱仪和软件，用于重叠荧光发射信号的明显分离; Zeiss LSM 510和Axiovision软件，用于快速信号处理和分析。蔡司510显微镜将安装在基础科学的中央显微镜核心设施中，并在那里进行监督和维护。该应用程序由大学的兴趣，需求和财政支持的广泛基础支持，并将服务于一个大型NIH资助的科学界。

DESCRIPTION (provided by applicant): We request a Zeiss LSM 510-Meta confocal microscope for sensitive fluorescence imaging of a variety of cells and tissues. The microscope will serve 8 major users and at least 50 minor users from multiple departments throughout the Johns Hopkins University Medical School. The users require a microscope that provides maximum sensitivity and spatial resolution, minimum crosstalk and bleedthrough, high speed and throughput, flexibility of raster scanning and wavelength analysis for FRET and FRAP, and robust software for image processing and analysis. The features of the Zeiss 510 confocal include: multitracking technology for speed and precise pixel localization; a Meta multi-channel spectrometer and software for distinct separation of overlapping fluorescence emission signals, and Zeiss LSM 510 and Axiovision software for rapid signal processing and analysis. The Zeiss 510 microscope will be installed in a centralized microscope core facility in the basic sciences where it will be supervised and maintained. The application is supported by a broad base of interest, need, and financial support by the university and will serve a large NIH-funded scientific community.