Perkin Elmer Ultraview Spinning Disc Confocal Microscope

描述(由申请人提供)：这是一份资金申请，购买Perkin Elmer Ultraview RS-3旋转圆盘共聚焦显微镜系统，配备徕卡DM IRE2电动倒置显微镜。该仪器将被安置在加州大学旧金山分校的解剖学系。这一多用户设施将为用户群提供三激光光学成像系统的通道，主要针对活细胞、胚胎和有机物以及较长时间内完整的活动物进行快速、实时、三色荧光成像。随着绿色和其他彩色荧光蛋白标记分子、量子微点和其他荧光标记试剂的功能蛋白质组学方法成为高质量研究的基准，对活细胞和动物成像仪器的需求非常迫切。目前，加州大学旧金山分校有许多传统的共焦和多光子显微镜，它们具有成像深度，其中一些具有三色能力，但它们不适合这些用途。该系统的重要方面是(A)成像深度，而不是成像速度，这是细胞快速移动所需的，(B)可靠性，(C)易用性，(D)一次对大片样本进行自动成像，以便能够捕捉随机事件，(E)在成像的较长时间(20分钟至3天)内极低的光漂白和光毒性，以及(F)较低的使用和维护成本。不存在类似的工具。 由6名调查人员组成的核心用户小组拥有加州大学旧金山分校帕纳苏斯校区美国国立卫生研究院提供的14多笔赠款，并在一系列领域取得了令人印象深刻的成就。作为校园成像核心的一部分，在可用时间的基础上，显微镜将向其他用户提供。购买所要求的仪器将使个人用户能够获得最先进的活细胞和动物成像技术，从而提高其个人赠款的生产率。

DESCRIPTION (provided by applicant): This is a request for funds to purchase a Perkin Elmer Ultraview RS-3 spinning disc confocal microscope system, with a Leica DM IRE2 motorized inverted microscope. The instrument will be housed in the Department of Anatomy, University of California, San Francisco. This multi-user facility will provide access for the user group to a three laser optical imaging system for rapid, real-time, three-color fluorescent imaging primarily for living cells, embryos and organoids, and intact live animal over extended periods of time. There is a critical need for instruments for live cell and animal imaging as functional proteomic approaches with green and other color fluorescent protein-tagged molecules, quantum microdots and other fluorescent -tagged reagents become the benchmark for high quality research. Currently there are a number of conventional confocal and multiphoton microscopes on the University of California, San Francisco that have the depth of imaging and several have three color capability, but they are not appropriate for these uses. The important aspects of this system are (a) not imaging depth, but imaging speed, which is needed for rapid cell movements, (b) reliability, (c) ease of use, (d) automated imaging of large areas of the sample at one time so that stochastic events can be caught, (e) very low photobleaching and phototoxicity over extended periods of imaging (20 min to 3 days) and (f) lower cost of use and maintenance. No comparable instrument exists. The core user group of six investigators has more than 14 grants from the National Institutes of Health on the Parnassus campus of University of California, San Francisco and has an impressive track record of accomplishments in a range of fields. As a part of a campus wide imaging core, on a time available basis the microscope will be made available to other users. The acquisition of the requested instrument will allow the individual users access to state-of-the-art live cell and animal imaging technology and thus enhance the productivity of their individual grants.