Core--Molecular and Neuropathology

该核心将为项目提供基因分型支持和神经病理学专业知识。尸检组织的确定和采集将在核心B中完成，组织处理和组织分析在核心C中完成。帕金森综合征患者的脑尸检和组织捐赠对于确认帕金森病或相关疾病的诊断至关重要，也是项目II表达研究的材料来源。为了确保高质量的研究材料，最好在死亡后尽快获得人脑组织，目前对于我们前瞻性招募的参与者，这种死后延迟平均不到10小时。这个核心将继续之前Udall PDRCE核心C，神经病理学核心的非常成功的工作。将进行组织学和病理学诊断，以及各种组织切片的储存和编目。使用Arcturus Autopix的激光捕获显微镜技术也将与 项目二.此外，该核心将为所有项目提供基因分型支持。Taqman引物和探针的设计和优化将在每个项目中进行。这使得SNP的具体工作可以在项目研究员的监督下进行。这些引物将输入核心C，核心C将进行实际的常规基因分型，其方案对于所有SNP基本相同。

This core will provide both genotyping support and neuropathology expertise to the program project. Ascertainment and collection of the autopsy tissue will be accomplished in Core B, with tissue processing and analysis of tissue in Core C. Brain autopsy and tissue donation from patients with Parkinsonism are essential for confirming the diagnosis of Parkinson Disease or related disorders, and as a source of material for the expression studies of Project II. To ensure high quality material for investigations, human brain tissue is preferably obtained as rapidly after death as possible, currently for our prospectively enrolled participants this post-mortem delay is averaging less than 10 hours. This core will continue the very successful work of the previous Udall PDRCE Core C, The Neuropathology Core. Histopathology and pathologic diagnosis will be performed, as well as storage and cataloging of various tissue sections. Laser capture microscope techniques using the Arcturus Autopix will also be performed, in collaboration with Project II. In addition, this core will provide genotyping support for all the projects. The design and optimization for Taqman primers and probes will occur in each project. This allows the SNP specific work to be performed under supervision of the project fellows. These primers will feed into Core C, which will perform the actual routine genotyping, whose protocols are essentially identical for all SNPs.