Core--Vector

载体核心将生产基于单纯疱疹病毒(HSV)的扩增子和逆转录病毒载体，用于Giffard和Steinberg的项目。将产生表达铜锌超氧化物歧化酶(SOD1)、锰超氧化物歧化酶(SOD2)、谷胱甘肽过氧化物酶(GPX)、抗氧化剂基因组合p35和crmA以及bcl2的载体。所有的载体都将是“双顺反子”的，既表达研究中的基因，又表达报告基因。对于每个实验载体，同源对照载体将包含有问题的基因，并在其中心插入一个终止密码子，以确保不表达。将产生表达SOD1、SOD2、过氧化氢酶、GPX和抗氧化剂基因组合的逆转录病毒载体。对照载体将表达报告基因β-半乳糖苷酶或目的基因的终止密码子版本。病媒核心的持续目的是确保为研究小组持续供应病媒，确保病媒生产过程中的质量控制，并解决病媒使用中遇到的问题。

The vector core will produce herpes simplex virus (HSV)-based amplicon and retroviral vectors to be used in Projects by Giffard and Steinberg. Vectors will be produced expressing the genes for CuZn-superoxide dismutase (SOD1), manganese SOD (SOD2), glutathione peroxidase (GPX), combinations of the antioxidant genes, p35 and crmA and bcl-2. All the vectors will be "bicistronic," expressing both the gene under investigation and a reporter gene. For each experimental vector, the cognate control vector will contain the gene in question with a stop codon inserted in its center, insuring non-expression. Retroviral vectors to express SOD1, SOD2, catalase, GPx and combinations of the antioxidant genes will be produced. Control vectors will express either the reporter gene beta-galactosidase or a stop codon version of the gene of interest. The continuing purposes of the Vector Core are to ensure a constant supply of vectors for the research groups, insure quality control in the production of the vectors, and to troubleshoot problems encountered in the use of the vectors.