Improvement of University of Oregon Animal Research Facilities

描述（由申请人提供）：我们的目标是扩大和翻新俄勒冈大学（UO）的核心动物设施，以提高PHS资助研究的生产力和影响，促进研究动物、研究人员和动物护理人员的安全和福利，并扩大全基因组研究的低温保存库。UO是使用鱼类模型研究脊椎动物发育、遗传学、基因组学、进化和遗传对人类疾病的贡献的先驱。由于这方面的专业知识，UO拥有斑马鱼国际资源中心（ZIRC）。UO已经开始将这些模型的发现转化为哺乳动物，并有一个雄心勃勃的小鼠遗传学项目，涉及新员工和现有的初级和高级教师。UO研究项目的成功给斑马鱼、棘鱼和老鼠设施以及ZIRC带来了严重的压力。我们建议通过建设、改造和设备购买的协同计划来缓解这种压力，包括创新的可持续设计策略，以增加能源节约。(1)我们建议在我们的斑马鱼设施新建、改造和购买新的养殖设备。这将增加我们可以维护的线路数量，提高工作流程效率。(2)我们建议对现有空间进行改造，并购买新设备，建立一个新的棘鱼设施。新设施将补充我们现有的非常小的设施，该设施拥有全球唯一的近交系，增加我们可以维护的品系数量，并改善工作流程。我们建议为UO正在建设的大楼购买新的鼠标设施设备。这将增加我们可以维持的细胞系的数量，减轻老鼠的压力，并使一些高级教员能够扩大他们的研究项目，包括老鼠。(4)我们建议为ZIRC购买新的冷冻保存设备。这些设备将被安置在UO在ZIRC附近建造的一栋大楼里。最近的技术突破使研究人员能够对斑马鱼的每个基因进行突变和功能注释。新设备将允许ZIRC从大量的遗传和基因组资源中冷冻保存精子。

DESCRIPTION (provided by applicant): Our goal is to expand and renovate core animal facilities at the University of Oregon (UO) to increase productivity and impact of PHS funded research, promote safety and welfare of research animals, researchers, and animal care staff, and expand a cryopreservation repository for genome-wide research. The UO is a pioneer in using fish models for research in vertebrate development, genetics, genomics, evolution, and genetic contributions to human disease. Because of this expertise, UO houses the Zebrafish International Resource Center (ZIRC). UO has begun to translate discoveries made in these models into mammals, with an ambitious program in mouse genetics, involving new hires and current junior and senior faculty. The success of UO research programs has put serious pressure on the Zebrafish, Stickleback, and Mouse Facilities, and on ZIRC. We propose to alleviate this pressure by a synergistic program of construction, renovation, and equipment purchase, including innovative sustainable design strategies to increase energy savings. (1) We propose new construction, renovation, and purchase of new aquaculture equipment in our Zebrafish Facility. This will increase the number of lines we can maintain and improve work flow efficiency. (2) We propose renovation of existing space and purchase of new equipment to establish a new Stickleback Facility. The new facility will complement our very small existing facility that houses the only inbred lines worldwide, increase the number of lines we can maintain, and improve work flow. (3) We propose to purchase equipment for a new Mouse Facility in a building UO is constructing. This will increase the number of lines we can maintain, alleviate stress on the mice, and enable several senior faculty to expand their research programs to include mice. (4) We propose to purchase new cryopreservation equipment for ZIRC. This equipment will be housed in a building UO is constructing adjacent to ZIRC. Recent technological breakthroughs are enabling researchers to mutate and functionally annotate every zebrafish gene. The new equipment will allow ZIRC to cryopreserve sperm from the extensive genetic and genomic resources created by this effort.