The role of benedict in Drosophila cell cycle regulation

描述（由申请人提供）：我的实验室的长期目标是提高我们对果蝇发育过程中细胞周期调节的理解。本提案的重点是benedict （bene）的分子鉴定和功能表征，该基因是我在博士后期间进行的克隆筛选中发现的。三种已知的bene功能丧失突变是致命的，并破坏了幼虫脂肪体和唾液腺的内复制，bene生殖系克隆表现出护士细胞染色体凝聚缺陷和护士细胞内复制的破坏。这些克隆的卵母细胞染色体形态具有减数分裂分裂缺陷的特征，有益的突变不是细胞致命的，也不会引起明显的有丝分裂缺陷。这一系列的bene缺陷表明bene可能在内复制和减数分裂细胞周期调节中发挥着独特的作用，并且在卵母细胞中破坏M期调节、S期调节和翻译调节的突变都表现出不同的bene表型亚群。我们已经绘制了一个包含31个候选基因的300 kb的基因组序列区域。

DESCRIPTION (provided by applicant): My laboratory's long-term goal is to improve our understanding of cell cycle regulation during Drosophila development. This proposal focuses on the molecular identification and functional characterization of benedict (bene), a gene identified in a clonal screen I carried out as a post-doctoral fellow. The three known loss-of-function mutations in bene are lethal and disrupt endoreplication in the larval fat body and salivary gland, bene germ line clones exhibit nurse cell chromosome condensation defects and disruption of nurse cell endoreplication. The morphology of oocyte chromosomes in these clones is characteristic of meiotic disruption defects, bene mutations are not cell lethal and do not cause obvious mitotic defects. The suite of bene defects suggests that bene may play a unique role in endoreplication and meiotic cell cycle regulation, and mutations that disrupt M phase regulation, S phase regulation, and translation regulation in oocytes all exhibit different subsets of the bene phenotypes. We have mapped bene to a 300 kb, sequenced region of the genome that includes 31 candidate genes. Specific Aim 1: To identify which of the 31 candidate genes in the bene region is disrupted in the mutant bene alleles and to determine the bene expression pattern\ Specific Aim 2: To analyze bene function in the larva and ovary by analysis of cell cycle markers and by assays for genetic interaction with cell cycle regulators.