Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe

光系统II（PSII）的最大光化学效率（Maximum photochemical efficiency of photosystem II，Fv/Fm），又称PSII的最大量子产额（maximum quantum yield of PSII，Fv/Fm），已成为浮游植物研究中应用最广泛的荧光参数之一。它代表潜在的光化学效率，这是由光合机构捕获的光能被用作光化学的概率。Fv/Fm已被证明对物理和化学性质的变化具有即时响应，并被解释为浮游植物整体健康或能力的诊断。与PSII的吸收截面积和叶绿素浓度一起，它可用于测量初级生产力（Cheah等人，2011年，深海研究）。<br /> <br /> 3米深的海水从船上的清洁海水管线连续供应。在暗适应状态下以1分钟间隔连续测量FRR荧光产率（！15分钟暗适应），使用由一系列100次欠饱和闪光（1.1 μs闪光持续时间和2.8 μs闪间周期）和一系列20次弛豫闪光（1.1 μs闪光持续时间和51.6 μs闪间周期）组成的闪光序列。

Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).<br /> <br /> Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).