A Large Scale Generation and Analysis of Mice Carrying *

描述(由申请人提供)： 哺乳动物肾脏的发育是一个非常复杂的过程，涉及输尿管的分支形态发生和肾的形成。一旦肾翼形成，下一个发育阶段就是Henle环和集合管延伸到肾盆，形成髓质。阐明肾脏基因的时空表达模式对于了解肾脏的器官发生和生理功能是至关重要的。因此，我们建议分析100个基因在肾脏发育过程中的表达模式。这些基因要么是新的，要么没有报道在肾脏中表达或发挥作用。我们将用绿色荧光蛋白标记这些基因，以可视化时空表达模式。对这些小鼠的分析将使我们能够：1)确定基因功能丧失对肾脏发育的影响；2)跟踪纯合子突变细胞的命运，因为它们现在被GFP标记；以及3)通过可视化GFP标签来确定基因产物的亚细胞定位，这通常是非常揭示基因功能的

DESCRIPTION (provided by applicant): The development of the mammalian kidney is a very complex process, involving branching morphogenesis of the ureter and the formation of nepherons. Once the nepheron is formed, the next stage of development is for the loop of Henle and collecting duct to elongate into the renal pelvis, forming the medulla. Delineation of the spatial and temporal expression patterns of genes in kidney is essential for understanding its organogenesis and physiological function. Therefore, we proposed to analyze the expression patterns of 100 genes in the developing kidney. These genes are either novel or have not been reported to be expressed or to be functioning in the kidney. We will tag these genes with GFP to visualize the spatio-temporal expression patterns. Analyses of these mice will be enable us to: 1) determine the effect of the loss of the gene function on renal development; 2) follow the fate of the homozygous mutant cells as they are now marked by GFP; and 3) determine the subcellular localization of the gene product by visualizing the GFP tag, which often time is very revealing about a gene's function