Neoparamoeba perurans: its unique microbiome and Impact on Amoebic Gill Disease and Gill Health in salmon Impact on Amoebic Gill Disease and Gill He

假设AGD的严重程度与新巴米巴与其细菌之间的复杂关系有关，特别是细胞内(最丰富的)弧菌本身的影响目的了解Perurans微生物群是否影响宿主-病原体的相互作用这将通过分析多个分离株并从周围环境中检测到弧菌来验证弧菌与Perurans的关联。这些数据将导致创建一张地图，将细菌的存在与轮虫的存在联系起来，结果将导致假设的发展-弧菌物种可能是AGD爆发的哨兵，以及其他参数，如季节性、水温。此外，计划使用鳃内单层系统测试弧菌在Perurans疾病挑战中的存在，以调查弧菌的存在对Perurans疾病严重程度和毒力因素的影响。

Hypothesisthe severity of AGD is associated with the complex relationship between Neoparamoeba and its bacteria and in particular, the influence of the intracellular (and most abundant) Vibrio species, a pathogen itselfAimto understand whether the N. perurans microbiome influences host-pathogen interactionsThis will be done by verifying that Vibrio species are associated with N. perurans, through the analysis of multiple isolates and detect them from the surrounding environments. This data will lead to the creation of a map, linking presence of bacteria to presence of N. perurans where the results should lead to the development of hypothesis - Vibrio species can be sentinels of AGD outbreaks, along with other parameters e.g. seasonality, water temperature. Also, it is planned to test the presence of vibrio on perurans disease challenges using the in-gill monolayer system, to investigate Vibrio presence on disease severity and virulence factors of N.perurans.