Exhaled Air Biomarkers in COPD

描述（由申请人提供）： COPD是一个世界性的健康问题，患病率不断增加。 疾病表型、疾病严重程度和进展速率的非侵入性标志物、表征COPD恶化的标志物是迫切需要的。 我们建议分析超滤呼出的空气样本，建立最佳的方案，样本收集后的FEV 1机动，调查脂质，包括磷脂，类花生酸，类固醇，核苷，蛋白质和肽作为潜在的疾病（活动）的标志物。 我们建议建立定量质谱技术（使用稳定同位素稀释协议）的目标标记物测定。 我们建议研究稳定的COPD患者在补充连续流氧疗之前和期间、α-l-抗胰蛋白酶输注治疗之前和期间、戒烟之前和之后以及肺移植之前和之后。

DESCRIPTION (provided by applicant): COPD is a world-wide health problem of increasing prevalence. Non-invasive markers of disease phenotype, severity and rate of progression of the disease, markers characterizing exacerbations of COPD are badly needed. We propose to analyze ultrafiltered exhaled air samples, to establish optimal protocols for sample collection following a FEV1 maneuver, to survey lipids including phospholipids, eicosanoids, steroids, nucleosides, proteins and peptides as markers of potential disease (activity). We propose to establish quantitative mass spectroscopic techniques (using stable isotope dilution protocols) for target marker assays. We propose to investigate stable COPD patients before and during supplemental continuous flow oxygen treatment, before and during alpha-l-antitrypsin infusion therapy, before and after smoking cessation and before and after lung transplantation.