High Throughput LSPR Nanasensor for Hormone Detection

描述(申请人提供)：随着婴儿潮一代的成熟，越来越多的女性进入更年期。虽然这是一种正常的生活转变，但它既有令人不快的(潮热)副作用，也可能有危险的(骨质流失)副作用。在许多女性中，更年期激素的变化早在症状出现之前就开始了。对于早期干预目的，高通量监测体液中的雌激素和其他激素水平是非常重要的。它还可以作为一种工具，通过分析和关联激素水平，为乳腺癌和与生殖有关的癌症的风险提供流行病学证据。智能光学系统公司(IOS)计划开发一种创新的、便携式的、灵敏的、高通量的传感器，用于快速识别和分析性类固醇激素。该传感器将基于局部表面等离子共振(LSPR)增强的受体结合技术。雌激素将被选为我们的模型系统。荷尔蒙的分析将使用连接到多路成像系统的三角形银纳米板进行。这种无标签检测将使流行病学激素水平的高通量和低成本监测具有更高的灵敏度。拟议的技术有可能扩展到医药、环境监测、生物技术以及药品和食品监测等领域的许多其他重要分析目标。

DESCRIPTION (provided by applicant): As the baby boomer generation matures, more and more women are reaching menopause. Although this is a normal life transition, it has both unpleasant (hot flashes) and possibly dangerous (bone loss) side effects. In many women, menopausal hormone changes begin long before symptoms appear. For early intervention purposes, the high throughput monitoring of estrogens and other hormone levels in body fluids is very important. It is also important as a tool for providing epidemiological evidence of the risks of breast cancer and reproductive related cancers through the analysis and correlation of hormone levels. Intelligent Optical Systems, Inc. (IOS) proposes to develop an innovative, portable, sensitive, high throughput sensor for the rapid recognition and analysis of sex steroid hormones. This sensor will be based on local surface plasmon resonance (LSPR)-enhanced receptor binding technology. Estrogen will be chosen as our model system. Assays of hormones will be conducted using triangular silver nanoplates that will be coupled to a multiplexed imaging system. This label-free detection will enable the high throughput and low cost monitoring of epidemiological hormone levels with improved sensitivity. The proposed technology has the potential to be extended to numerous other important analytical targets in areas such as medicine, environmental monitoring, biotechnology, and drug and food monitoring.