Controlled Release Bone Morphogenic Protein/Nano-calcium

描述(申请人提供)：使用纳米磷酸钙涂层控制释放骨形态发生蛋白TP-2004-71我们提出了一种在金属植入物上沉积基于纳米相磷酸钙和骨形态发生蛋白(BMP)混合物的载药涂层的新方法。该涂层的一个独特特征是，它将在外科手术后以受控的方式释放药物。此外，涂层由纳米级的磷酸钙组成，我们在初步研究中已经证明，这可以显著增强成骨细胞的功能。在第一阶段的可行性研究中，普渡大学将合成三种纳米钙磷酸盐涂层配方(非晶态、半晶态和晶态)。然后，纳米相磷酸钙将与骨形态发生蛋白混合，并使用我们的新沉积工艺涂覆在钛衬底上。将对这些样品上的涂层进行释放速率评估，目的是重现我们之前从颗粒状纳米CaPCVBMP材料中获得的成功结果。在控制释放速率被证实后，我们将在体外用成骨细胞反应性实验评估含有和不含BMP的纳米钙磷酸盐材料的蛋白质生物活性。利用扫描电子显微镜、X射线衍射仪、原子力显微镜和能谱仪对涂层样品的各种结构和材料特性进行了研究。在第二阶段，植入物上的纳米CaPCVBMP涂层将在体内动物模型中进行评估。

DESCRIPTION (provided by applicant): Controlled Release of Bone Morphogenic Protein using Nano-Calcium Phosphate Coatings TP-2004-71 We propose a novel method for depositing a drug-loaded coating based on a mixture of nano-phase calcium phosphate and bone morphogenic protein (BMP) on metal implants. One unique feature of the coating is that it will release the drug in a controlled fashion following the surgical procedure. Additionally, the coating is made up of nano-sized calcium phosphate features, which we have shown in preliminary studies to significantly enhance osteoblast function. In Phase I feasibility studies, three nano-calcium phosphate coating formulations (amorphous, semi- crystalline and crystalline) will be synthesized at Purdue University. Nano-phase calcium phosphate will then be mixed with BMP and coated onto titanium substrates using our novel deposition process. The coating on these samples will be evaluated for rate of release with the goal of reproducing our successful previous results from pelletized form of nano-CaPCVBMP material. After controlled rate of release has been demonstrated, we will assess protein bioactivity in vitro using osteoblast reactivity assay on nano-calcium phosphate material with and without BMP. Various structural and material characteristics of the coated samples will be studied using SEM, XRD, AFM and EDS. In Phase II, nano-CaPCVBMP coatings on implants will be evaluated in an in vivo animal model.