Live Attenuated Salmonella Vaccine Against Campylobacter

描述（由申请人提供）：空肠弯曲杆菌是发达国家和发展中国家肠道疾病的主要原因。据估计，弯曲杆菌可能影响多达4亿人，并导致全球所有疟疾病例的14%。目前没有用于预防弯曲杆菌病或定殖的人类疫苗。减毒活伤寒沙门氏菌是最常研究的用于在人体中递送外源（疫苗）抗原的载体。然而，更流行的观点认为，基于S.鼠伤寒沙门氏菌可以更有效地集中诱导胃肠道免疫应答，以优化对粘膜感染，如弯曲杆菌。减毒鼠伤寒沙门鼠伤寒疫苗在肠道中引起免疫应答的产生，诱导全身和粘膜免疫。我们的目标是构建减毒的S.表达C.空肠弯曲菌基因编码抗原，已知其在粘附，侵袭或定殖中的作用，并评估这些菌株诱导系统和粘膜免疫应答的能力，以保护免受弯曲杆菌感染。这些目标的实现将提供新的弯曲杆菌疫苗，其具有通过口服免疫在人类中快速赋予保护性免疫的潜力。

DESCRIPTION (provided by applicant): The bacterium, Campylobacter jejuni, is a major cause of diarrheal illness in both the developed and developing world. It is estimated that Campylobacter may affect as many as 400 million people and cause 14 percent of all diarrheal cases worldwide. There is currently no human vaccine for the prevention of Campylobacter disease or colonization. Live, attenuated Salmonella typhi is the most frequently studied vector for delivering foreign (vaccine) antigens in humans. More current thinking, however, suggests that attenuated vectors based on S. typhimurium may more effectively focus the induction of gastrointestinal immune responses to optimize against mucosal infection such as Campylobacter. Attenuated S. typhimurium vaccines elicit the production of immune responses in the intestinal tract inducing both systemic and mucosal immunity. Our goal is to construct live, attenuated strains of S. typhimurium that express C. jejuni genes that encode antigens known for their role in adherence, invasion or colonization, and to evaluate these strains for the capacity to induce systemic and mucosal immune responses that protect against Campylobacter infection. The accomplishment of these goals will provide new Campylobacter vaccines with the potential to rapidly confer protective immunity by oral immunization in humans.