Core--IMMUNODEFICIENT MOUSE COLONY

免疫缺陷小鼠核心设施的目的是为该项目的研究人员提供生物模型，以测试体内前列腺癌的致瘤性。严重联合免疫缺陷（SCID）小鼠人肿瘤异种移植模型将是这些研究中采用的主要模型，但也可使用其他动物模型，如无胸腺裸鼠模型。SCID小鼠异种移植模型测试肿瘤生长、扩散、渗透、局部扩散和远处转移。具体的可测量终点是定性和定量的。定性评估包括大体解剖学以及组织学和人类前列腺异种移植物的免疫拓扑学的表型描述。定量评估包括肿瘤异种移植物的大小、数量、浸润深度和动物存活数据。已经开发了利用异位以及原位注射人前列腺细胞系的模型， 这一核心将以下列方式支持项目： 1)提供开展SCID小鼠-人肿瘤异种移植实验的技术专长。 2)协助设计和解释利用人前列腺肿瘤生长和转移的SCID小鼠模型的实验。 3)根据项目0001、0002和0005所述，提供来自SCID小鼠人异种移植物的固定、冷冻和新鲜组织进行分析。

The purpose of the immunodeficient mouse core facility is to serve the investigators in this program project by providing them with access to biological models to test the tumorigenicity of prostate carcinoma in vivo. The severe combined immune-defcient (SCID) mouse human tumor xenograft model will be the predominant model employed in these studies but other animal models such as the athymic nude mouse model may also be utilized. The SCID mouse xenograft models test tumor growth, adhesiveness, penetration, local spread and distant metastases. The specific measurable end points are both qualitative and quantitative. Qualitative assessments include gross anatomic as well as histologic and phenotypic descriptions of the immunotopography of human prostate xenografts. Quantitative assessment includes tumor xenograft size, number, depth of invasion, and animal survival data. Models have been developed utilizing both ectopic as well as orthotopic injections of human prostate cell lines, This core will support the projects in the following manner: 1) Provide the technical expertise to perform the SCID mouse-human tumor xenograft experiments. 2) Assist with the design and interpretation of experiments utilizing SCID mouse models of human prostate tumor growth and metastasis. 3) Provide fixed, frozen, and fresh tissue from the SCID mouse human xenografts for analysis as described in projects 0001, 0002 and 0005.