Washington University Center for Translational Neuroscience

华盛顿大学翻译神经科学中心(WUCTN)的光学成像核心将 允许神经科学界的成员扩展已经成功的项目并发起新的 项目，特别是在生物发光领域。虽然目前有各种光学成像 医疗中心和主校区内的设施，这些都没有提供给更大的 神经科学界，因为他们只有有限的技术支持。为了实现其目标， CORE将协调华盛顿大学现有的共焦和双光子资源 通过神经科学家的显微成像，还建立了新的微光成像能力。访问 光学成像核心设施将扩展到华盛顿大学神经科学家在三个不同的 地点。两个设施将设在医学院(WUMS)，一个设在本科生 校园(吴)。所有这三个设施都将为不同的显微镜方法提供指导和监督 支持。光学成像核心将提供以下设施： 1.用于荧光探针高分辨率成像的共聚焦显微镜。这包括成像 以及GFP变体(如YFP、CFP、EGFP)和更多红移染料(Cy5)在 包括活的和固定的准备。 2.用于深部组织成像的双光子显微镜，包括体内和体外时间序列测量。 使用双光子技术将把重复成像造成的损害降到最低。 3.荧光和生物发光记者的微光成像。这包括实时成像 使用荧光素酶构建的基因活性和使用新型转基因记者的其他细胞事件的活性 在培养的细胞、组织切片和整个小动物中。 4.为神经科学家提供技术支持，确保高效地创建高质量的图像 定量的图像分析，并最终出版。这包括培训、教育、软件 影像设备和材料的开发、数据管理和维护。 所描述的54个项目中有22个涉及与临床相关的研究，其他大多数涉及假说 这直接为翻译研究提供了信息。值得注意的是，有几个项目涉及新的实验方向 以及通过访问这些核心设施实现和促进的协作。

The Optical Imaging Core of the Washington University Center for Translational Neuroscience (WUCTN) will allow members of the Neuroscience community to expand already successful projects and initiate new projects, especially in the area of bioluminescence. While there are presently a variety of optical imaging facilities within the medical center and main campus, these have not been available to the larger neuroscience community because they have only limited technical support. To accomplish its goals, the Core will coordinate already existing resources at Washington University for confocal and two-photon microscopy by neuroscientists and also establish new low-light level imaging capabilities. Access to the Optical Imaging Core Facilities will be extended to Washington University neuroscientists at three separate locations. Two facilities will be housed at the Medical Campus (WUMS) and one at the Undergraduate campus (WU). All three facilities will offer instruction and supervision for the different microscopy methods supported. The Optical Imaging Core will offer facilities for: 1. Confocal microscopy for high resolution imaging of fluorescent probes. This includes the imaging and differentiation of variants of GFP (such as YFP, CFP, eGFP), and more red-shifted dyes (Cy5) in both living and fixed preparations. 2. Two-photon microscopy for deep tissue imaging, including in vivo and in vitro time-series measures. Use of the 2-photon technique will minimize damage due to repeated imaging. 3. Low-light imaging of fluorescent and bioluminescent reporters. This includes real-time imaging of gene activity using luciferase constructs and of other cellular events using novel transgenic reporters in cultured cells, in tissue slices and in whole small animals. 4. Technical support for the neuroscientists to ensure the efficient creation of high quality images for quantitative image analysis and ultimately publication. This includes training, education, software development, data management and maintenance of imaging equipment and materials. 22 of the 54 projects described involve clinically-relevant studies, and most of the others address hypotheses that directly inform translational research. Significantly several projects involve new experimental directions and collaborations that will be enabled and facilitated by access to these Core facilities.