CORE--FLOURESCENCE/ISOLATED MUSCLE

荧光/分离肌肉将为研究人员提供一套光度学、高分辨率荧光成像、分离心肌设备和专业知识，以促进拟议的实验。我们将利用与更广泛的约翰·霍普金斯研究社区共享的现有显微镜设施，以及建立 用于同时测量心脏小梁力和荧光的站，仅与研究人员共享。完整的细胞和组织的荧光成像将利用约翰霍普金斯大学的多光子显微镜设施进行，该设施也是由该机构指导的。 除了提供设备外，一个关键的支持功能将是培训和协助 研究人员在精细的肌肉分离和染料注射过程中。对于完整的肌肉 为了做好准备，一名训练有素的技术人员将在咨询一名研究缺血后心脏小梁肌肉表现的教职研究员的情况下协助实验，这名研究员在这项技术方面拥有多年经验。

The FLUORESCENCE/ISOLATED MUSCLE will make available to investigators a suite of photometry, high resolution fluorescence imaging, and isolated cardiac muscle equipment and the expertise to facilitate the proposed experiments. We will leverage existing microscopy facilities that are shared with the wider Johns Hopkins research community, as well as establish stations for the measurement of cardiac trabecula force and fluorescence simultaneously, to be shared exclusively with investigators. Fluorescence imaging of intact cells and tissues will be carried out utilizing the Johns Hopkins Multiphoton microscope facility, which is also directed. In addition to making the equipment available, a key support feature will be to train and assist investigators in the delicate procedure of muscle isolation and dye injection. For the intact muscle preparation, a well-trained technician will assist in the experiments, in consultation with a faculty investigator who has studied muscle performance in trabeculae from post-ischemic hearts and who has many years of experience in the technique.