Sensor for Paralytic Shellfish Poisons

描述（由申请人提供）：有害藻华，或“HAB”，每年与许多人类中毒病例有关。截至20世纪90年代末，渔业、公共卫生和旅游业每年的经济损失和成本估计为4000万美元。这种有害藻华会导致麻痹性贝类中毒（PSP）。在藻华之后，微生物释放的毒素可能会在滤食性贝类中积累，食用这些毒素会引起疾病症状，其中最严重的是呼吸麻痹和死亡。PSPs是少数几种海洋毒素之一，当人们从食物中摄入时，它们可能对人类致命。本提案寻求资金以发展最近报道的将psp荧光检测到荧光检测板的技术。在第一阶段，将确定4种PSPs的检出限，并对有毒贝类提取物的荧光响应进行初步评价。在第二阶段，这种板将用于便携式设备。本项目的具体目标是：1。合成适合于玻璃载玻片共价附着的荧光化学传感器。2. 传感器在载玻片上的自组装和共价附着以及表面性能和涂层的评价。3. 蛤蚌毒素、PSP毒素和有毒贝类提取液的荧光扩增评价。麻痹性贝类毒素（PSPs）最常通过滤食性贝类横切物进入人类食物链。毒性最大的PSP——蛤蚌毒素，也是政府“精选药剂”名单上仅有的3种“小分子”之一。需要一种快速、廉价的检测psp的方法。

DESCRIPTION (provided by applicant): Harmful Algal Blooms, or "HAB's", are linked to many cases of human poisoning each year. Economic losses and costs to the fishing industry, public health, and tourism are estimated to be $40,000,000 annually, as of the late 1990s. 1 such HAB causes paralytic shellfish poisoning (PSP). Following an algal bloom, the toxins released by the microbes may accumulate in filter-feeding shellfish, consumption of which then causes disease symptoms, the most severe of which is respiratory paralysis and death. PSPs are 1 of only a few marine toxins that can be fatal to humans when consumed in food. This proposal seeks funding to develop recently reported technology for the fluorescence detection of PSPs into fluorescent detector plates. In Phase I, the limits of detection of 4 PSPs will be determined, and a preliminary evaluation of fluorescence response in toxic shellfish extracts will be conducted. Such plates will be adapted for use in a portable device in Phase II. The Specific Aims of this project are: 1. Synthesize fluorescent chemosensors suitable for covalent attachment to a glass slide. 2. Self-assembly and covalent attachment of the sensors to glass slides and evaluation of the surface properties and coating. 3. Evaluation of the fluorescence amplification to solutions of saxitoxin, PSP toxins, and toxic shellfish extracts. Paralytic Shellfish Poisons (PSPs) enter the human food chain most commonly through filter-feeding shellfish transvectors. The most toxic PSP, saxitoxin, is also 1 of only 3 "small molecules" on the government's list of "Select Agents". Fast, inexpensive means of detection PSPs is desirable.