Developing and applying fluorescent nanosensors for biological measurement.

荧光纳米传感器是一种惰性、多功能的生物传感器，可用于在生命物理界面上对微环境中的关键分子和离子进行重要测量，并且非常适合动态过程的实时测量[1]。在诺丁汉大学，荧光纳米传感器已被开发用于定量复杂模型系统中的pH、[2]分子氧[3]和温度[4]。在这些传感器中，pH敏感的荧光纳米传感器获得了最大的动力。它们已经在一系列复杂和多样化的微环境中进行了评估和验证，并通过绘制线虫模式生物(秀丽线虫[5]和Pristionchus pacphaus[6])的酸化图谱、阐明酿酒酵母的亚细胞发酵途径[7]、确定人间充质干细胞(HMSCs)内异物的处理[8]以及表征细菌生物膜生长过程中酸性副产物的演变[9]来展示其巨大的潜力。该项目将继续将pH敏感的荧光纳米传感器应用于不同的生物环境，包括果蝇(Droflila Blackogaster)、Tale cress(Arabiopsis Thaliana)和CHO/Heck 293细胞培养用于生物加工。以及开发用于关键生物分子和离子的新型生物传感器。

Fluorescent nanosensors are inert, versatile biosensors, that can be used to make important measurements of key molecules and ions in microenvironments at the physics of life interface and are ideal for real-time measurements of dynamic processes [1].At the University of Nottingham, fluorescent nanosensors have been developed to quantify pH, [2] molecular oxygen [3] and temperature [4] in complex model systems. Of these sensors, the pH-sensitive fluorescent nanosensors have gathered the greatest momentum. They have been evaluated and validated in a range of complex and diverse microenvironments and demonstrated their immense potential by mapping the acidification in nematode model organisms (Caenorhabditis elegans [5] & Pristionchus pacificus [6]), elucidation of subcellular fermentation pathways in Saccharomyces cerevisiae [7], determined the intracellular processing of foreign material in human mesenchymal stem cells (hMSCs) [8] & characterised the evolution of acid by-products during bacterial biofilm growth [9].This project will continue the application of pH-sensitive fluorescent nanosensors to diverse biological environments, which include fruit flies (Drosophila melanogaster), Thale cress (Arabidopsis thaliana) and CHO/Heck 293 cell culture for bioprocessing. As well as develop new biosensors for key biological molecules and ions.