Einstein Center for Human Embryonic Stem Cell Research

描述（由申请人提供）：拟议的爱因斯坦人类胚胎干细胞研究中心的总体目标将是推进人类胚胎干细胞（hESCs）的基础生物学知识，并创建一个机构基础设施，以促进阿尔伯特·爱因斯坦医学院及其附属机构的科学界获得hesc相关技术。为了实现这些目标，将建立一个中央细胞培养核心设施，为研究人员提供无差别的hESCs，为hESCs和试剂提供定期质量控制服务，并为教师，博士后和学生提供培训。一个行政核心将帮助管理赠款，并将协调中心将提供的讲习班、研讨会系列和其他培训活动。该设施的最初使用者将是四个试验性项目，每个项目都处理一个重要的基本生物学问题。项目1将研究hESCs中的DNA甲基化和增殖。项目2将描述CDKS和CDKiS在控制hESCs生长和分化中的作用。项目3将研究hESCs中的DNA复制和核定位，项目4将分析hESCs中的遗传网络。我们建议在此应用中使用的hESC线是WA01， WA09和UC06。

DESCRIPTION (provided by applicant): The overall aims of the proposed Einstein Center for Human Embryonic Stem Cell Research will be to advance knowledge of the fundamental biology of human embryonic stem cells (hESCs) and to create an institutional infrastructure that will facilitate access to hESC-related technologies by the scientific community at the Albert Einstein College of Medicine and its affiliated institutions. To achieve these goals, a central cell culture core facility that will provide undifferentiated hESCs to investigators, periodic quality control services for hESCs and reagents, and training for faculty, post-docs and students will be established. An administrative core will help administer the grant and will coordinate the workshop, seminar series and other training activities that will be provided by the Center. Four pilot projects each addressing an important fundamental biological question will be the initial users of the facility. Project 1 will examine DNA methylation and epimutation in hESCs. Project 2 will characterize the role of CDKS and CDKiS in the control of growth and differentiation of hESCs. Project 3 will look at DNA replication and nuclear localization in hESCs and Project 4 will analyze genetic networks in hESCs. The hESC lines that we propose to use in this application are WA01, WA09 and UC06.