Genetic Regulation of Telencephalon Development

描述（由申请人提供）：本提案的中心原则是， 同源异型盒基因的Dlx家族对于调节 前脑GABA能神经元的分化和功能。因此我 表明Dlx基因是控制发育所必需的， 也许是大脑皮层中间神经元的功能， 基底神经节的神经元。因此，了解Dlx的功能 基因应该有助于了解前脑GABA能神经元的作用， 神经系统，并可能提供洞察前脑疾病，由于 GABA能神经元的缺陷。本文所述的实验使用标准和 条件遗传学方法来改变Dlx基因的表达， 发育中的老鼠这些方法将使我们能够研究删除 单个和/或多个Dlx基因对发育和功能的影响 前脑GABA能神经元。此外，我们还将研究 异位表达Dlx基因。这些研究对 了解调控该规范的遗传途径， 前脑GABA能神经元的分化和功能，并具有 对理解人类疾病的分子基础的影响 GABA能神经传递。

DESCRIPTION (provided by applicant): The central tenet of this proposal is that the Dlx family of homeobox genes is essential for regulating the differentiation and function of forebrain GABAergic neurons. Accordingly, I suggest that the Dlx genes are required for controlling the development, and perhaps the function, of intemeurons in the cerebral cortex, and projection neurons in the basal ganglia. Therefore, understanding the functions of the Dlx genes should yield insights into the roles of GABAergic neurons in forebrain neural systems, and may provide insights into disorders of the forebrain due to defects in GABAergic neurons. The experiments described herein use standard and conditional genetic methods to alter the expression of the Dlx genes in developing mice. These methods will allow us to study the effects of deleting individual and/or multiple Dlx genes on the development and function of forebrain GABAergic neurons. In addition, we will investigate the effects of ectopically expressing the Dlx genes. These studies have implications for understanding the genetic pathways that regulate the specification, differentiation and function of forebrain GABAergic neurons, and have implications for understanding the molecular bases of human disorders of GABAergic neurotransmission.