Array-microscope Multispectral Whole Slide Imager

描述（由申请人提供）：项目摘要/摘要：我们建议开发一种创新的“多对一”的光学系统，用于全载玻片多光谱载玻片扫描仪，以快速生成病理学中的虚拟载玻片。该光学系统的主要用途是能够对组织和组织微阵列载玻片进行多光谱成像。多光谱成像是推进基于组织的病理学分析的诊断和预后的关键发展。多谱段成像技术起源于遥感技术。在显微镜的背景下，它仍然是一个最近的发展。在遥感和显微镜中，多光谱成像的主要优点是分别对光谱重叠的矿物或分子特征进行数字解混。这里提出的光学系统将允许扫描显微镜载玻片在一个单一的扫描在NA = 0.65的数值孔径，覆盖整个宽度和长度的载玻片与0.47微米的采样。此外，建议的多光谱幻灯片扫描仪将提供至少16个连续的光谱带测量在每个（0.47微米）2像素的整个幻灯片图像。所有多光谱数据将在10秒内收集，比现有的全载玻片扫描仪快5倍或更多倍，后者只能捕获每个标本的3种颜色。目前，市场上没有同等产品。第一阶段项目强调仪器原型的设计和组装。原型的评价强调图像质量，光学传输效率和收集的多光谱数据的准确性。将与我们的2个合作者获得的多光谱成像结果的一致性与原型进行比较。在第二阶段，我们计划开发一个模块化的，“工业”阵列显微镜仪器快速多光谱成像，适用于明场和落射荧光成像模式。项目叙述：多光谱技术与DALBO的超快速自动成像技术相结合，代表了推进诊断和预后组织病理学分析的关键发展。手术病理学家将使用多光谱成像技术来确定肿瘤病因、多参数测定和病理学以及治疗药物决策。

DESCRIPTION (provided by applicant): Project Summary/Abstract: We propose to develop an innovative "many-to-one" optical system for a whole-slide multispectral slide scanner to rapidly generate virtual slides in pathology. A primary use of this optical system is to enable multispectral imaging of tissue and tissue-microarray slides. Multispectral imaging is a key development in advancing diagnostic and prognostic tissue-based pathology analysis. Multispectral imaging has its roots in remote sensing. In the context of microscopy, it is still a recent development. The main advantage of multispectral imaging, in remote sensing as well as in microscopy, is the digital unmixing of spectrally overlapping mineral or molecular signatures, respectively. The optical system proposed here will allow scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.47-um sampling. In addition, the proposed multispectral slide scanner will deliver at least 16 contiguous spectral-band measurements at each (0.47 um)2 pixel in the whole-slide image. All multispectral data will be collected in 10's of seconds, 5 or more times faster than existing whole-slide scanners which only capture 3 colors per specimen. At this time, there is no equivalent product on the market. The Phase I project emphasizes design and assembly of an instrument prototype. The evaluation of the prototype emphasizes image quality, optical transfer efficiency, and accuracy of collected multispectral data. Measurements taken with the prototype will be compared for consistency with multispectral-imaging results obtained by our 2 collaborators. In Phase II, we plan to develop a modular, "industrial" array-microscope instrument for rapid multispectral imaging, applicable in brightfield and epi-fluorescence imaging modalities. Project Narrative: Multispectral technology, combined with Dmetrix's ultra-rapid, automated imaging technology, represents a key development in advancing diagnostic and prognostic tissue-based pathology analysis. Multispectral imaging technology will be used by the surgical pathologist to define tumor etiology, multiparameter assays and prognostics as well as therapeutic drug decisions.