Single-Molecule Imaging Studies of the Polyomaviruses

描述（由申请人提供）： 单分子成像提供了一种直接观察多瘤病毒感染的方法，这是不可能使用传统的整体技术。除SV40外，多瘤病毒的感染机制尚不清楚。已经表明，鼠多瘤病毒的内化独立于网格蛋白或小窝。我们建议使用单分子成像技术来表征小鼠多瘤病毒和人多瘤病毒JCV的内化和内吞途径。MPV和JCV的单分子成像研究将表征病毒感染所需的广泛的内吞途径。与SV40和流感的比较将提供对病毒感染的广泛理解，这对于开发抗病毒药物至关重要。

DESCRIPTION (provided by applicant): Single-molecule imaging provides a method for the direct observation of polyomavirus infection that is not possible using conventional, ensemble, techniques. The infection mechanisms of the polyomaviruses, with the exception of SV40, are not well-understood. It has been suggested that the murine polyoma virus is internalized independently of clathrin or caveolae. We propose to use single-molecule imaging techniques to characterize the internalization and endocytic pathways of the murine polyoma virus and the human polyomavirus, JCV. Single-molecule imaging studies of MPV and JCV will characterize the broad range of endocytic pathways necessary for viral infection. Comparisons to SV40 and influenza will provide a broad understanding of viral infection that will be essential in the development of antiviral drugs.