Purchase of a New confocal Zeiss LSM 510 v3.2

描述（由申请人提供）：本次重新提交的总体目标是通过购买新的蔡司倒置LSM 510 v3.2来提高我们当前共聚焦设备的研究能力。现有的共聚焦设备（LSM 510 v1.6）于1998年购买，目前是医学院唯一的共聚焦设备。虽然它主要由主要用户组使用，但它可供整个波士顿大学医学院社区使用，并且已经使用或超过容量并且正在老化。我们的目标是购买一台新的共聚焦显微镜，这将扩大设备规模，并允许所有研究人员使用新技术。v1.6版本将继续保留，并将作为教学工具，供有常规需要的用户和有溢出需要的用户使用。由于我们目前的容量超过了容量，升级不会使其他成员受益，而是允许大学拥有更大的用户群。我们要求安装在Axiovert 200M上的v3.2 LSM 510，带有LSM #18 405/AR/Dual HENE/3 PMT和4个内部探测器通道。我们还要求一个机动化的舞台，以支持目前正在进行的大量现场和固定研究。生理学，多时间序列和3D分析软件对于支持研究人员的需求也至关重要，因为它们已经发展并包括具有多个触发点的Ca2+成像。该名单还包括那些与我联系但不能使用当前仪器的研究人员，因为它不支持他们的实验需求。在现有的显微镜上收集了大量的数据，用户现在受到技术的限制和它已经达到极限的事实的限制。主要用户定期进行活细胞成像实验，以评估发育或跟踪对损伤的反应。医学院院长支持这台仪器作为大学的设施，并据此支持一名全职人员每天运行共聚焦。Dean McCahan还支持服务合同和支持共聚焦系统和工作站所需的院系。

DESCRIPTION (provided by applicant): The overall goal of this resubmission is to increase the research capabilities of our current confocal facility with the purchase of a new Zeiss inverted LSM 510 v3.2. The existing confocal (LSM 510 v1.6) was purchased in 1998 and is currently the only confocal facility on the medical campus. While it is used mainly by the major user group it is made available to the entire Boston University Medical School Community and is used to or over capacity and is aging. Our goal is to purchase a new confocal microscope that will expand the facility and allow for new technologies available to all investigators. The v1.6 will be maintained and will serve as a teaching instrument, be available for users with conventional needs and for overflow needs. As we are currently above capacity an upgrade would not benefit additional members and allow for a larger user base for the university. We are requesting a v3.2 LSM 510 mounted on an Axiovert 200M with the LSM #18 405/AR/Dual HENE/3 PMT and 4 internal detector channels. We are also requesting a motorized stage to support a large number of live and fixed studies that are currently performed. The Physiology, multi-time series and 3D analysis software are also critical to support the investigators needs as they have evolved and include Ca2+ imaging with several trigger points. The list also includes investigators who have approached me but can not use the current instrument as it does not support their experimentation needs. Extensive data has been collected on the existing microscope and users are now being constrained by the limitations of the technology and the fact that it is used to capacity. The major users routinely perform live cell imaging experiments over time to assess development or follow the response to injury. The Dean of the Medical School supports this instrument as the facility for the University and in accordance with that supports a full time person to run the confocal on a daily basis. Dean McCahan also supports the service contracts and the faculties that are needed to support the confocal systems and work station.