Laser Scanning Confocal Microscope

描述(申请人提供)：购买激光扫描共聚焦显微镜需要资金。该系统，蔡司LSM 510 Meta与Axiovert 200 MOT相结合，将取代已有10年历史的LSM 410系统，拥有来自29个部门的232名注册用户。申请的系统将由我们大学最古老的成像核心设施神经科学成像中心(NIC)容纳和管理。NIC聘请了一名拥有8年LSM 410共焦显微镜操作经验的全职技术人员。描述了来自8个主要用户和9个次要用户的项目。这些项目将受益于LSM 510 Meta改进的光学和检测系统，从而改进对氟铬发射的捕获、光谱分离、降低光毒性和进行荧光共振能量转移(FRET)测量的能力。主要使用者的项目包括：大鼠和小鼠海马片或培养物中的钙离子通道运输(Herlitze博士)，肌球蛋白II在人类癌细胞移动过程中的作用和机械功能(Egelhoff博士)，作为脑5-羟色胺能神经元研究的遗传工具的新小鼠品系的开发(Deneris博士)，5-羟色胺受体靶向和运输的调节(Roth博士)，动力相互作用蛋白在黑腹果蝇细胞内周期中的作用(Robinson博士)，内吞作用在EGF受体运输中的作用(Carlin博士)，出生后小脑分化的调节(西格尔博士)和神经胶质疤痕的轴突再生(西尔弗博士)。

DESCRIPTION (provided by applicant): Funds are requested to purchase a laser scanning confocal microscope. This system, the Zeiss LSM 510 Meta coupled to an Axiovert 200 MOT, will replace a 10-year-old LSM 410 system with 232 registered users from 29 departments. The requested system will be housed and administered by the Neuroscience Imaging Center (NIC), the oldest imaging core facility at our University. The NIC employs a full-time technician with 8 years of experience operating the LSM 410 confocal microscope. Projects from 8 major and 9 minor users are described. These projects will benefit from the improved optics and detection system of the LSM 510 Meta, resulting in improved capture of fluorochrome emission, spectral separation, reduced phototoxicity and the ability to do fluorescence resonance energy transfer (FRET) measurements. Projects of the major users include: Ca 2+ channel trafficking in hippocampal slices or cultures of rat and mice (Dr. Herlitze), role and mechanical function of myosin II during cell locomotion in human cancer cells (Dr. Egelhoff), development of new mouse strains as genetic based tools for brain serotonergic neuron studies (Dr. Deneris), regulation of 5-HT-receptor targeting and trafficking (Dr. Roth), role of dynamin interacting proteins in the endocytotic cycle in Drosophila melanogaster (Dr. Robinson), role of endocytosis in EGF receptor trafficking (Dr. Carlin), regulation of postnatal cerebellar differentiation (Dr. Siegel) and axon regeneration across glial scar (Dr. Silver).