Nano-HPLC-ESI Quadrupole Ion Trap Mass Spectrometer

描述（由申请人提供）：我们申请资金购买用于蛋白质和蛋白质组分析的电喷雾电离离子阱质谱仪。具体的仪器是一个Thermo Finnigan LTQ线性四极离子阱质谱系统，耦合到一个能够100 nL到uL流速的Surveyor HPLC系统和一个纳米或电喷雾源。罗彻斯特大学没有类似的仪器。有8个主要用户和至少2个次要用户证明需要这种仪器，所有这些用户都是由NIH资助的。Alan V. Smrcka（首席研究员）一直在使用氢/氘交换MALDI-TOF质谱法分析信号转导蛋白。获得这种仪器将大大提高这种分析的水平。其他主要用户建议使用该系统研究酵母中的全局蛋白修饰（E. Phizicky），参与组蛋白核转运的蛋白（J. Hayes），果蝇中转录因子相互作用的分析（D. Bohmann），参与组蛋白转运的蛋白(J。Hayes)，神经退行性疾病的蛋白质组学分析（D. Pearce）， G蛋白偶联受体的翻译后修饰（P. Hinkle），人类唾液蛋白质组学的综合分析（J. Melvin），以及四膜虫的微管蛋白和组蛋白修饰（M. Gorovsky）。LC/ESI离子阱质谱仪将被整合到功能基因组学核心设备中，该设备还包含MALDI-TOF和微阵列仪器。硕士水平的质谱师将负责仪器的日常操作。一个质谱委员会已经成立，其中包括一名外部顾问成员，他将指导范德比尔特大学的一个质谱核心设施，该设施将为该仪器的使用提供长期指导。机构承诺将为硕士级质谱师提供三年的工资支持，并将支付三年的服务合同。将实施一项成本回收计划，以支付未来几年的成本。

DESCRIPTION (provided by applicant): We request funds for the purchase of an electrospray-ionization ion trap mass spectrometer for protein and proteome analysis. The specific instrument is a Thermo Finnigan LTQ linear quadrupole ion trap mass spectrometry system coupled to a Surveyor HPLC system capable of 100 nL to uL flow rates and a nano or electrospray source. No instrument with comparable capabilities is available at the University of Rochester. There is a need for this instrument as demonstrated by eight major users and at least two minor users, all of whom are funded by NIH. Alan V. Smrcka (Principal Investigator) has been using hydrogen/deuterium exchange MALDI-TOF mass spectrometry for analysis of signal transduction proteins. Acquiring this instrumentation would greatly advance the level of this analysis. Other major users propose to use the system to study global protein modifications in yeast (E. Phizicky), proteins involved in nuclear transport of histones (J. Hayes), analysis of transcription factor interactions in Drosophila (D. Bohmann), proteins involved in histone transport (J .Hayes), proteomic analysis ofneurodegenerative diseases (D. Pearce), Posttranslational modifications of G protein coupled receptors (P. Hinkle), comprehensive analysis of the human salivary proteome (J. Melvin), and tubulin and histone modifications in Tetrahymena (M. Gorovsky). The LC/ESI ion trap mass spectrometer will be incorporated into a functional genomics core facility that also contains a MALDI-TOF and microarray instrumentation. A Masters level mass spectrometrist will be responsible for the day to day operation of the instrument. A Mass Spectrometry Committee has been named, that includes an outside advisory member who directs a mass spectrometry core facility at Vanderbilt University that will provide long-term guidance about the use of the instrument. The institutional commitment will provide salary support for the Masters level mass spectrometrist for three (3) years and will pay the service contract for three (3) years. A cost recovery plan will be implemented that will cover costs in future years.