Molecular and Genomic Imaging Center

描述：(由申请人提供)我们在此建议分子和基因组成像中心(MGIC)，以响应生物医学社区对灵活、经济高效、高分辨率技术的需求，以在基因组和转录水平上识别和表征生物系统的变异。我们计划通过开发聚合酶菌落或Polony技术来帮助满足这一需求。Polonies代表了一种高度并行的核酸分析方法，它逼真、近在咫尺、模块化和多功能。MGIC的主要使命是有效地将技术开发人员的各种贡献整合到一个强大的平台中，该平台可以顺利地传播给具有特殊临床和生物兴趣的各种用户。主要目标有：(1)高度平行的荧光原位测序。(2)转录组的单分子图谱，特别是神经分化和哺乳动物选择性剪接。(3)直接分子单倍型和远程序列连通性。(4)单细胞DNA和RNA的鉴定，特别是哺乳动物干细胞不对称分裂的鉴定。(5)解决核酸分析中组合模式和空间模式的计算算法和系统建模。(6)ELSI组成部分侧重于将技术转化为临床应用的问题以及对匿名概念的挑战。

DESCRIPTION: (provided by applicant) We propose here the Molecular and Genomic Imaging Center (MGIC) in response to a biomedical-community-wide need for flexible, cost-effective, high-resolution technology to identify and characterize variation in biological systems at the level of genomes and transcriptomes. We plan to help meet this need by developing the polymerase colony, or polony, technology. Polonies represent a highly parallel method of nucleic acid analysis that is realistic, close-at-hand, modular, and versatile. The primary mission of the MGIC is to efficiently integrate a diverse set of contributions from technology developers into a robust platform that can be smoothly disseminated to a variety of users with specialized clinical and biological interests. These are the main aims: (1) Highly parallel fluorescent in situ sequencing. (2) Single molecule profiling of the transcriptome, in particular of neural differentiation and of mammalian alternative splicing. (3) Direct molecular haplotyping and long-range sequence connectivity. (4) Characterization of DNA & RNA from single cells, in particular characterizing asymmetric cell division in mammalian stem cells. (5) Computational algorithms and systems modeling addressing combinatorial and spatial patterns in nucleic acid analysis. (6) An ELSI component focuses on issues of translation of technology to clinical applications and challenges to the concepts of anonymity.