Animal Core

核心B是一个动物核心，将由道格拉斯·A·库尔特执导，并将每天进行管理 由玛丽·艾伦·凯利博士提供的依据。核心还将涉及两名高级技术员的努力：沙林 纳尔逊和艾丽西亚·怀特。这个核心将产生用于这个实验的所有动物 PROAM项目拨款提案，包括立即出现匹罗卡品诱发的癫痫持续状态的动物 (MOSS提案，项目3)，癫痫持续状态后1-21天的动物(ALL项目)，以及慢性癫痫 动物，癫痫持续状态后1-3个月(所有项目)。所有项目都需要有经验的MICE 匹罗卡品致癫痫持续状态。所有的实验都要求小鼠在行为上或 对癫痫发作活动进行脑电监测。为了将潜在的可变性降到最低 实验室，因为正确实施这一癫痫模型的所有方面需要大量的 在动物护理人员的投资下，我们选择了开发一个集中的核心，负责我们所有的 动物的需要。 具体来说，动物核心将负责毛果芸香碱诱导的SE、SE后的护理、长时间的 对我们的慢性小鼠进行长期视频或视频/EEC监测，以确认是否存在脑电 和/或行为性癫痫、插管和注射病毒和药物，以及所有 立体定向手术。 癫痫研究的最大障碍之一是缺乏充分的、有能力的 准备了这种疾病的动物模型。通过将所有提案的动物模型生成结合在一起 将项目赠款纳入一个核心设施，由动物繁殖和表征方面的专家负责 对于癫痫模型，这应有助于提案内的所有研究。此外，此核心将扩展 计划项目中的所有调查人员进行研究的能力，其中候选机制可以 操纵，并对癫痫发生的后果进行评估。长期视频/脑电监测是一项 专门的、费力的、困难的和昂贵的事业，全国几乎没有实验室能够 有能力进行这样的研究。向所有调查人员提供这一专家动物监测能力 使项目1、2和3中的实验超越了细胞和电路的表征，以及 实际评估整个动物对癫痫发病率和严重程度的影响。这是马上 扩大了所有这些计划的翻译影响。

Core B is an Animal Core, which will be directed by Douglas A. Coulter, and will be managed on a day to day basis by Dr. Mary Ellen Kelly. The Core will also involve the efforts of two Senior Technicians: Shareen Nelson and Alicia White. This core will generate all of the animals to be utilized in experiments in this Progam Project Grant proposal, including animals immediately in pilocarpine-induced status epilepticus (Moss proposal, Project 3), animals 1-21 days after status epilepticus (AllProjects), and chronically epileptic animals, 1-3 months after status epilepticus (AllProjects). All projects will require mice that experience pilocarpine-induced status epilepticus. and all experiments require mice to be behaviourally or electrographically monitored for seizure activity. In order to minimize potential variability between laboratories and because proper implementation of all aspects of this epilepsy model requires substantial investment by animal care personnel, we have chosen to develop a centralized core responsible for all our animal needs. Specifically the animal core will be responsible for induction of pilocarpine-induced SE, post-SE care, long- term video or video/EEC monitoring of our chronic mice in order to confirm the presence of electrographic and/or behavioural seizures, cannula implantation and administration of viruses and drugs, and all stereotaxic surgeries. One of the biggest impediments in epilepsy research is lack of adequately characterized, competently prepared animal models of this disorder. By combining animal model generation for all proposals within this Program Project Grant into one core facility, manned by experts in generation and characterization of animal models of epilepsy, this should facilitate all studies within the proposal. Additionally, this core will extend the ability of all investigators in the Program Project to conduct studies in which candidate mechanisms can be manipulated, and consequences on epileptogenesis assessed. Long-term video/EEG monitoring is a specialized, laborious, difficult and expensive undertaking, with few laboratories in the country able to competently conduct such studies. Providing this expert animal monitoring capability to all investigators enables experiments in Projects 1, 2 and 3 to move beyond cellular and circuit characterizations, and actually assess effects in whole animals, on the incidence and severity of epilepsy. This immediately expands the translational impact of all of these programs.