Rigaku/MSC High-Throughput HomeLab x-ray crystallography system

描述（由申请人提供）：弗雷德哈钦森癌症研究中心的结构分子生物学项目寻求对我们的共享内部X射线晶体学设施进行重大升级，以保持其存在的过去十多年中实现的高质量研究和生产力水平。此次升级将用最先进的系统（理学/MSC高性能家庭实验室）取代老化的设备，最大限度地利用当前设施中仍然有用的设备。此升级解决了3个主要问题：1）更换故障（12年以上）图像板探测器（R-AXIS Me），很快将失去制造商的支持; 2）大幅扩展当前系统的能力，以允许以前需要使用我们有限的同步加速器时间的内部实验; 3）增加内部数据收集吞吐量，以减轻目前饱和设施的等待时间，并促进主要用户在不久的将来大幅扩展个人研究计划。哈钦森的结构分子生物学计划包括3个主要用户（Ferre-D 'Amare，Stoddard，Strong），同时还支持3个次要用户（Cronk，Hockenbery，麦克法兰），其中2人是前哈钦森博士后，他们在缺乏X射线设备的本科院校开始了独立的职位。各个研究项目是多样的，但高度互补和充分整合，在结构酶学领域产生高影响力的结果（Ferre-D 'Amare，Cronk，Stoddard），结构分子免疫学（麦克法兰，强），核酶功能和RNA拓扑结构（Ferre-D 'Amare），内含子编码的限制性内切核酸酶（Stoddard）、细胞凋亡（Hockenbery）、基于结构的药物设计（Hockenbery，Stoddard）以及开创蛋白质设计和结构分析新方法的发展（Stoddard，Strong）。该计划还支持非常成功的博士前和博士后培训计划，与4个独立的研究生课程（分子和细胞生物学（FHCRC/UW），生物化学（UW），生物分子结构与设计（UW）和免疫学（UW））接口。研究和培训计划的深度和广度不断增长，越来越需要升级的X射线仪器，这是必要的，以防止生产力的严重损失，由于不可修复的故障，我们的2个图像板探测器中的一个（无支持的R-AXIS IIc）。

DESCRIPTION (provided by applicant): The Structural Molecular Biology program of the Fred Hutchinson Cancer Research Center seeks a major upgrade of our shared, in-house x-ray crystallography facility in order to maintain the high quality of research and level of productivity achieved over the past decade-plus of its existence. This upgrade would replace aging equipment with a state-of-the-art system (a Rigaku/MSC High-Throughput HomeLab), maximally leveraging still useful equipment in the current facility. This upgrade addresses 3 major concerns: 1) Replacement of a failing (12+ years) image plate detector (R-AXIS Me) that is soon to lose manufacturer support; 2) Dramatically expanding the capability of the current system to allow in-house experiments previously requiring use of our limited synchrotron time; 3) Increasing the in-house data collection throughput to relieve wait-times on a currently saturated facility and facilitate dramatic expansions of the individual research programs of the Major Users in the near future. The Hutchinson's Structural Molecular Biology program comprises 3 major users (Ferre-D'Amare, Stoddard, Strong) while also supporting 3 minor users (Cronk, Hockenbery, McFarland), 2 of whom are ex-Hutchinson post-docs that have started independent positions at undergraduate institutions lacking x-ray equipment. The individual research programs are diverse, but highly complementary and fully integrated, generating high-impact results in the fields of structural enzymology (Ferre-D'Amare, Cronk, Stoddard), structural molecular immunology (McFarland, Strong), ribozyme function and RNA topology (Ferre-D'Amare), intron-encoded restriction endonucleases (Stoddard), apoptosis (Hockenbery), structure-based drug design (Hockenbery, Stoddard) and pioneering the development of new methods in protein design and structure analysis (Stoddard, Strong). The program also supports a highly successful pre-and post-doctoral training program, interfacing with 4 separate graduate programs (Molecular and Cellular Biology (FHCRC/UW), Biochemistry (UW), Biomolecular Structure & Design (UW) and Immunology (UW)). The growing depth and breadth of the research and training programs increasingly require upgraded x-ray instrumentation and are necessary to prevent a crippling loss in productivity with the anticipated, eventual loss, due to an unrepairable breakdown, of 1 of our 2 image plate detectors (the unsupported R-AXIS IIc).